Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E-64 | complete inhibition of all five ficin isoforms at 0.1 mM | Ficus carica | |
iodoacetamide | complete inhibition of all five ficin isoforms at 5 mM | Ficus carica | |
methylmethanethiol sulfonate | complete inhibition of all five ficin isoforms at 3 mM | Ficus carica | |
additional information | all five ficin isoforms are not inhibited by pepstatin A, Pafabloc SC, and EDTA | Ficus carica | |
Nalpha-tosyl-L-lysine chloromethyl ketone | complete inhibition of all five ficin isoforms at 0.1 mM | Ficus carica | |
Nalpha-tosyl-L-phenylalanine chloromethylketone | complete inhibition of all five ficin isoforms at 0.1 mM | Ficus carica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ficus carica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SP-Sepharose column chromatography | Ficus carica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Ficus carica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.044 | - |
purified isoform ficin A, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.046 | - |
purified isoform ficin B, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.073 | - |
purified isoform ficin C, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.11 | - |
purified isoform ficin D2, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.117 | - |
purified isoform ficin D1, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.127 | - |
purified isoform ficin A, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.146 | - |
purified isoform ficin D1, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.154 | - |
purified isoform ficin D2, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.186 | - |
purified isoform ficin C, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
0.204 | - |
purified isoform ficin B, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C | Ficus carica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O | - |
Ficus carica | Nalpha-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide + H2O | - |
Ficus carica | tert-butyloxycarbonyl-L-Ala-L-Ala-Gly + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * around 24000, native enzyme, SDS-PAGE | Ficus carica |
Synonyms | Comment | Organism |
---|---|---|
ficin A | isoform | Ficus carica |
ficin B | isoform | Ficus carica |
ficin C | isoform | Ficus carica |
ficin D1 | isoform | Ficus carica |
ficin D2 | isoform | Ficus carica |