Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Grb2-associated binding protein 1 + H2O | Coxsackievirus B3 | GAB1 is cleaved during CV-B3 infection by viral proteinase 2A | ? | - |
? | |
Grb2-associated binding protein 2 + H2O | Coxsackievirus B3 | GAB2 is cleaved at G238 during CV-B3 infection by viral proteinase 2A, generating two cleaved fragments of GAB2-N1-237 and GAB2-C238-676 | GAB2-N1-237 + GAB2-C238-676 | - |
? | |
additional information | Coxsackievirus B3 | in vivo assays by expression of GAB2 proteins in virus-infected HeLa cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coxsackievirus B3 | - |
CV-B3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the virus is propagated in HeLa cells | Coxsackievirus B3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Grb2-associated binding protein 1 + H2O | GAB1 is cleaved during CV-B3 infection by viral proteinase 2A | Coxsackievirus B3 | ? | - |
? | |
Grb2-associated binding protein 2 + H2O | GAB2 is cleaved at G238 during CV-B3 infection by viral proteinase 2A, generating two cleaved fragments of GAB2-N1-237 and GAB2-C238-676 | Coxsackievirus B3 | GAB2-N1-237 + GAB2-C238-676 | - |
? | |
Grb2-associated binding protein 2 + H2O | GAB2 is cleaved at G238 during CV-B3 infection by viral proteinase 2A, generating two cleaved fragments of GAB2-N1-237 and GAB2-C238-676. mutant GAB2G238E is non-cleavable for the enzyme | Coxsackievirus B3 | GAB2-N1-237 + GAB2-C238-676 | - |
? | |
additional information | in vivo assays by expression of GAB2 proteins in virus-infected HeLa cells | Coxsackievirus B3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CV-B3 proteinase 2A | - |
Coxsackievirus B3 |
proteinase 2A | - |
Coxsackievirus B3 |
General Information | Comment | Organism |
---|---|---|
malfunction | a catalytic mutant of 2A (2Amut) fails to cleave GAB2. Deletion of GAB1 causes a decrease in phosphorylated ERK1/2, but knockdown of GAB2 has no effect on virus-mediated ERK1/2 phosphorylation | Coxsackievirus B3 |
physiological function | Grb2-associated binding protein 2 (GAB2) is cleaved at G238 during CV-B3 infection by viral proteinase 2A. Knockdown of GAB2 significantly inhibits the synthesis of viral protein and subsequent viral progeny production, accompanied by reduced levels of phosphorylated p38, suggesting a pro-viral function for GAB2 linked to p38 activation. Expression of the cleavage products of GAB2 does not further enhance viral replication, indicating that GAB2 cleavage results in its loss-of-function, rather than gain-of-function in supporting viral replication that represents a distinct host defense mechanism | Coxsackievirus B3 |