Protein Variants | Comment | Organism |
---|---|---|
M27G | indistinguishable from wild type enzyme | Enterovirus C |
M27G/C147S | 430fold reduction in specificity rate constant, the bell-shaped curve for the modified enzyme is shifted towards the alkaline pH values by 0.45 units on the acidic side and by 0.9 units on the alkaline side. The reduced activity of the enzyme is primarily due to the less ordered ground state of ist reaction | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoic acid-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Phe(NO2)-Ala + H2O | - |
Enterovirus C | ? | - |
? | |
2-aminobenzoic acid-Phe-Thr-Gln-Ser-Glu-Gly-Glu-Phe(NO2)-Ala + H2O | poor substrate | Enterovirus C | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
pH 8.0, melting point of mutant enzyme M27G/C147S is 46.5, for mutant enzyme M37G it is 46.7°C | Enterovirus C |
48 | - |
pH 8.5, melting point of mutant enzyme M27G/C147S is 47.7°C, for mutant enzyme M27G it is 48.1°C | Enterovirus C |
50 | - |
pH 9.5, melting point of mutant enzyme M27G/C147S and M27G is 50.4, for mutant enzyme M27G it is 50.0°C | Enterovirus C |