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Literature summary for 3.4.22.28 extracted from

  • Schultheiss, T.; Kusov, Y.Y.; Gauss-Muller, V.
    Proteinase 3C of hepatitis A virus (HAV) cleaves the HAV polyprotein P2-P3 at all sites including VP1/2A and 2A/2B (1994), Virology, 198, 275-281.
    View publication on PubMed
Search for more literature for 3.4.22.28

General Stability

General Stability Organism
enzyme is less stable in eukaryotic cells than in bacteria Hepatovirus A

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant enzyme Hepatovirus A
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hepatitis A virus polyprotein + H2O Hepatovirus A involved in primary cleavages yielding precursors of structural proteins, it mediates all secondary cleavages yielding mature structural and functional proteins ?
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 ?

Organism

Organism UniProt Comment Textmining
Hepatovirus A
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hepatitis A virus deltaVP1-P2-P3 polyprotein fragment + H2O putative primary cleavage sites within P2 region are VP1-2A, 2A/2B, i.e. Gln745-Ser and Gln836-Ala Hepatovirus A hepatitis A virus proteins2BC + hepatitis A virus proteins P3* + hepatitis A virus proteins 2B + hepatitis A virus proteins deltaVP1-2A + hepatitis A virus proteins 2A
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 ?
hepatitis A virus P2-P3 precursor polyprotein + H2O trans-cleavage, rapid cleavage at P2/P3 junction, the 3A/3B, 3B/3C and 3C/3D sites are less efficiently cleaved, the hepatitis A virus enzyme has a broader activity profile than enzymes of other picornaviruses, recombinant enzyme cleaves all putative cleavage sites of the polyprotein Hepatovirus A ?
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 ?
hepatitis A virus polyprotein + H2O involved in primary cleavages yielding precursors of structural proteins, it mediates all secondary cleavages yielding mature structural and functional proteins Hepatovirus A ?
-
 ?
additional information the pattern of proteolytic products of polyprotein processing in recombinant systems and in infected cells differs, mature protease 3C is produced in bacteria, but cleavage is arrested at 3ABC intermediate in eukaryotic system Hepatovirus A ?
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 ?

pI Value

Organism Comment pI Value Maximum pI Value
Hepatovirus A isoelectric focusing
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 8.5