General Stability | Organism |
---|---|
enzyme is less stable in eukaryotic cells than in bacteria | Hepatovirus A |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant enzyme | Hepatovirus A | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hepatitis A virus polyprotein + H2O | Hepatovirus A | involved in primary cleavages yielding precursors of structural proteins, it mediates all secondary cleavages yielding mature structural and functional proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepatovirus A | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hepatitis A virus deltaVP1-P2-P3 polyprotein fragment + H2O | putative primary cleavage sites within P2 region are VP1-2A, 2A/2B, i.e. Gln745-Ser and Gln836-Ala | Hepatovirus A | hepatitis A virus proteins2BC + hepatitis A virus proteins P3* + hepatitis A virus proteins 2B + hepatitis A virus proteins deltaVP1-2A + hepatitis A virus proteins 2A | - |
? | |
hepatitis A virus P2-P3 precursor polyprotein + H2O | trans-cleavage, rapid cleavage at P2/P3 junction, the 3A/3B, 3B/3C and 3C/3D sites are less efficiently cleaved, the hepatitis A virus enzyme has a broader activity profile than enzymes of other picornaviruses, recombinant enzyme cleaves all putative cleavage sites of the polyprotein | Hepatovirus A | ? | - |
? | |
hepatitis A virus polyprotein + H2O | involved in primary cleavages yielding precursors of structural proteins, it mediates all secondary cleavages yielding mature structural and functional proteins | Hepatovirus A | ? | - |
? | |
additional information | the pattern of proteolytic products of polyprotein processing in recombinant systems and in infected cells differs, mature protease 3C is produced in bacteria, but cleavage is arrested at 3ABC intermediate in eukaryotic system | Hepatovirus A | ? | - |
? |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hepatovirus A | isoelectric focusing | - |
8.5 |