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Literature summary for 3.4.22.27 extracted from

  • Burden, R.E.; Snoddy, P.; Buick, R.J.; Johnston, J.A.; Walker, B.; Scott, C.J.
    Recombinant cathepsin S propeptide attenuates cell invasion by inhibition of cathepsin L-like proteases in tumor microenvironment (2008), Mol. Cancer Ther., 7, 538-547.
    View publication on PubMed
Search for more literature for 3.4.22.27

Cloned(Commentary)

Cloned (Comment) Organism
the cathepsin S propeptide is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cathepsin S propeptide 0.0002 mM, blocks gelatinase, collagenase and elastinase activities of mature cathepsin S Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 His-tagged recombinant propeptide, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ HiTrap column chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
complete removal of urea phosphate buffer starting from 8 M Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
Collagen + H2O
-
 Homo sapiens ?
-
 ?
Elastin + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
CatS mature enzyme Homo sapiens
CatSPP cathepsin S propeptide Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000025
-
 cathepsin S propeptide using benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin as a substrate Homo sapiens