Literature summary for 3.4.22.27 extracted from

Vasiljeva, O.; Dolinar, M.; Pungercar, J.R.; Turk, V.; Turk, B.
Recombinant human procathepsin S is capable of autocatalytic processing at neutral pH in the presence of glycosaminoglycans (2005), FEBS Lett., 579, 1285-1290.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21(DE3) in inclusion bodies	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	autocatalytic activation of procathepsin S, recombinant procathepsin S is capable of autocatalytic processing at neutral pH in the presence of about 20fold activating glycosaminoglycans, or polysaccharides, such as dextran sulfate, chondroitin sulfates A and E, and dermatan sulfate, activation via electrostatic interaction with procathepsin S, heparin, heparan sulfate, and hyaluronic acid have poor or no effect	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
solubilized and refolded recombinant enzyme	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
solubilization of recombinant enzyme from inclusion bodies after expression in Escherichia coli, refolding and reoxidizing	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	autocatalysis assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	autocatalysis assay at	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
3.5	9	pH profiles of autocatalytic processing activity in presence or absence of dermatan sulfate, overview	Homo sapiens

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Release 2023.1 (January 2023)