Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.27 extracted from

  • Brömme, D.; Bonneau, P.R.; Lachance, P.; Wiederanders, B.; Kirschke, H.; Peters, C.; Thomas, D.Y.; Storer, A.C.; Vernet, T.
    Functional expression of human cathepsin S in Saccharomyces cerevisiae. Purification and characterization of the recombinant enzyme (1993), J. Biol. Chem., 268, 4832-4838.
    View publication on PubMed
Search for more literature for 3.4.22.27

Protein Variants

Protein Variants Comment Organism
C25S inactive mutant enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Phe-Ala-CHN2
-
 Homo sapiens
benzyloxycarbonyl-Val-Val-Lys-NHO-4-nitrobenzoyl
-
 Homo sapiens
E-64
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 benzyloxycarbonyl-Leu-Leu-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
0.0121
-
 benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
0.0177
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
0.0208
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens
proteolytic modification the precursor can be activated in vitro at ph 4.5 and 40°C under reducing conditions. The in vitro activated enzyme has a NH2-terminal extension when compared with the native bovine enzyme. The precursor of cathepsin S can also be efficiently transformed into the mature enzyme by treatment with subtilisin BPN‘. The MW of the nonglycosylated precursor is 35900 Da Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7200
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Leu-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Leu-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin
-
 Homo sapiens benzyloxycarbonyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
butyloxycarbonyl-L-Phe-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens butyloxycarbonyl-L-Phe-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens tert-butyloxycarbonyl-Phe-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 above, inactivated within min Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 benzyloxycarbonyl-Leu-Leu-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
2
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
2.9
-
 benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
3.3
-
 butyloxycarbonyl-L-Phe-L-Phe-L-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens
14.4
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 reaction with benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
 benzyloxycarbonyl-Val-Val-Lys-NHO-4-nitrobenzoyl pH 6.5, 25°C Homo sapiens
0.00125
-
 Benzyloxycarbonyl-Phe-Ala-CHN2 pH 6.5, 25°C Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
 8.3