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Literature summary for 3.4.22.27 extracted from
- Sequence analysis, tissue distribution, and expression of rat cathepsin S (1992), J. Biol. Chem., 267, 26038-26043.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a glutathione S-transferase-fusion protein | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cystatin | 0.5 mg/ml, 82% inhibition | Rattus norvegicus | |
| E-64 | 0.005 mM, 96% inhibition | Rattus norvegicus |  |
| iodoacetic acid | 1 mM, 92% inhibition | Rattus norvegicus | |
| leupeptin | 1 mM, complete inhibition | Rattus norvegicus | |
| NEM | 1 mM, complete inhibition | Rattus norvegicus | |
| p-chloromercuriphenylsulfonic acid | 1 mM, 96% inhibition | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | cathepsin S is possibly involved in thyroid hormone biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the cDNA encodes a 330-residue protein with a 17-amino acid putative signal sequence, 95-residue ürpregion, and 216-residue mature protein | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | low activity | Rattus norvegicus | - |
| ileum | - |
Rattus norvegicus | - |
| jejunum | low activity | Rattus norvegicus | - |
| kidney | low activity | Rattus norvegicus | - |
| liver | low activity | Rattus norvegicus | - |
| lung | low activity | Rattus norvegicus | - |
| ovary | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| thyroid gland | - |
Rattus norvegicus | - |
| uterus | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| additional information | cathepsin S is possibly involved in thyroid hormone biosynthesis | Rattus norvegicus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
15 min, retention of more than 80% enzyme activity at pH 7.0, about 40% retention at pH 4.7 and about 50% retention at pH 8.5 | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | pH 5.5: about 45% of maximal activity, pH 8.5: about 50% of maximal activity, reaction with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
37°C, about 40% retention of activity after 15 min | Rattus norvegicus |
| 7 | - |
37°C, more than 80% retention of activity after 15 min | Rattus norvegicus |
| 8.5 | - |
37°C, about 50% retention of activity after 15 min | Rattus norvegicus |
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