Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl Phe-Ala-CH2F | very potent time-dependent inhibitor, inhibits proteinase 30times more efficiently than it does cathepsin L and about 300times better than it does cathepsin B | Bos taurus | |
benzyloxycarbonyl-Phe-Ala-CH2F | - |
Bos taurus | |
benzyloxycarbonyl-Phe-Lys(benzyloxycarbonyl)-CH3 | inhibition in the micromolar range, competitive | Bos taurus | |
benzyloxycarbonyl-Val-Val-Phe-CH3 | inhibition in the micromolar range, competitive | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
tert-butyloxycarbonyl-Phe-Leu-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
0.0081 | - |
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
0.0147 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
0.0175 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
0.0375 | - |
tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Val-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | the enzyme prefers small neutral amino acid residues in the subsites S2 and S3 | Bos taurus | ? | - |
? | |
Oxidized insulin B-chain + H2O | major cleavage sites: Glu13-Ala14, Leu17-Val18 and Phe25-Tyr26. Minor cleavage sites: Asn3-Gln4, Ser9-His10 and Leu15-Tyr16 | Bos taurus | ? | - |
? | |
tert-butyloxycarbonyl-Phe-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | tert-butyloxycarbonyl-Phe-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | tert-butyloxycarbonyl-Phe-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
6.1 | - |
tert-butyloxycarbonyl-Phe-Leu-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
8.6 | - |
tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
13 | - |
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus | |
40.5 | - |
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin | pH 6.5, 25°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | reaction with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000018 | - |
benzyloxycarbonyl-Phe-Ala-CH2F | pH 6.5 | Bos taurus | |
0.0014 | - |
benzyloxycarbonyl-Phe-Lys(benzyloxycarbonyl)-CH3 | pH 6.5 | Bos taurus | |
0.0018 | - |
benzyloxycarbonyl-Val-Val-CH3 | pH 6.5 | Bos taurus |