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Literature summary for 3.4.22.27 extracted from

  • Maciewicz, R.A.; Etherington, D.J.
    A comparison of four cathepsins (B, L, N and S) with collagenolytic activity from rabbit spleen (1988), Biochem. J., 256, 433-440.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Phe-Phe-CHN2
-
Oryctolagus cuniculus
cystatin
-
Oryctolagus cuniculus
Ep-475 0.01 mM, complete inhibition Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-7-amido-4-methylcoumarin + H2O no activity detected Oryctolagus cuniculus Arg + 7-amino-4-methylcoumarin
-
?
azocasein + H2O reaction only in presence of urea Oryctolagus cuniculus ?
-
?
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
-
Oryctolagus cuniculus benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Oryctolagus cuniculus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
Collagen + H2O
-
Oryctolagus cuniculus ?
-
?
Gelatin + H2O alpha-chain Oryctolagus cuniculus ?
-
?
additional information no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and benzoyl-Gly-Gly-7-amido-4-methylcoumarin Oryctolagus cuniculus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000000000583
-
gelatin alpha-chain Oryctolagus cuniculus
0.000000011
-
azocasein in presence of urea Oryctolagus cuniculus
0.000000548
-
Collagen alpha-chain Oryctolagus cuniculus
0.02
-
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin
-
Oryctolagus cuniculus
0.0283
-
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
Oryctolagus cuniculus

pI Value

Organism Comment pI Value Maximum pI Value
Oryctolagus cuniculus isoelectric focusing
-
6.8