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Literature summary for 3.4.22.25 extracted from

  • Buttle, D.J.; Ritonja, A.; Pearl, L.H.; Turk, V.; Barrett, A.J.
    Selective cleavage of glycyl bonds by papaya proteinase IV (1990), FEBS Lett., 260, 195-197.
    View publication on PubMed
Search for more literature for 3.4.22.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 tert-butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide
-
 Carica papaya
0.16
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide
-
 Carica papaya
5.2
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide
-
 Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Carica papaya
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tert-Butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O
-
 Carica papaya ?
-
 ?
tert-Butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide + H2O
-
 Carica papaya ?
-
 ?
tert-Butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide + H2O
-
 Carica papaya ?
-
 ?
Trypsin + H2O 16 cleavage points of which 13 are glycyl bonds Carica papaya Hydrolyzed trypsin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 tert-butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide
-
 Carica papaya
5
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide
-
 Carica papaya
22
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide
-
 Carica papaya