Cloned (Comment) | Organism |
---|---|
wild-type papain and D158N variant produced in a baculovirus insect cell expression system | Carica papaya |
Protein Variants | Comment | Organism |
---|---|---|
D158N | clear kinetic and pH depndence differences from the wild-type enzyme | Carica papaya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
benzyloxycarbonyl-L-Phe-L-Arg 4-methylcoumarin-7-amide | recombinant wild-type enzyme | Carica papaya | |
0.42 | - |
benzyloxycarbonyl-L-Phe-L-Arg 4-methylcoumarin-7-amide | commercial enzyme preparation | Carica papaya | |
1.8 | - |
benzyloxycarbonyl-L-citrullyl-L-Arg 4-methylcoumarin-7-amide | mutant enzyme D158N | Carica papaya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Carica papaya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-citrullyl-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Carica papaya | benzyloxycarbonyl-L-citrullyl-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Carica papaya | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide + H2O | - |
Carica papaya | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | recombinant wild-type enzyme | Carica papaya | |
52 | - |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | commercial enzyme preparation | Carica papaya |