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Literature summary for 3.4.22.2 extracted from
- Enzyme catalytic promiscuity: The papain-catalyzed Knoevenagel reaction (2012), Biochimie, 94, 656-661.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methyl methanethiosulfonate | - |
Carica papaya |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzaldehyde + acetylacetone | 35% yield after 72 h at 25°C or 55% yield after 81 h at 60°C. 150 mg of papain is the optimum quantity for the Knoevenagel reaction between 2 mM of benzaldehyde and 2.4 mM of acetylacetone in 5 ml of DMSO/H2O | Carica papaya | 3-benzylidenepentane-2,4-dione | - |
? | |
| casein + H2O | - |
Carica papaya | L-tyrosine + ? | - |
? | |
| Nalpha-benzoyl-L-arginine ethyl ester + H2O | - |
Carica papaya | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
Knoevenagel reaction | Carica papaya |
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