Crystallization (Comment) | Organism |
---|---|
a complex of chagasin, a protein inhibitor from Trypanosoma cruzi, and papain is crystallized by using the hanging-drop vapor diffusion method at 18°C, the high-resolution crystal structure shows an inhibitory wedge comprising three loops, which forms a number of contacts responsible for the high-affinity binding, the present chagasin-papain complex provides a reliable model of chagasin-cruzipain interactions | Carica papaya |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chagasin | protease inhibitor, inhibits all C1 family proteases | Carica papaya | |
cystatin B | - |
Carica papaya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
carboxybenzoyl-Phe-Arg-7-(4-methyl)coumarylamide | 100 mM sodium phosphate buffer, pH 6.0, containing 1 mM dithiothreitol and 1 mM EDTA | Carica papaya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Carica papaya | papain is the founding member of the large C1 family of papain-like cysteine proteases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | P00784 | - |
- |
Purification (Comment) | Organism |
---|---|
active papain is prepared from the commercial papaya latex enzyme through affinity purification on Sepharose 4B | Carica papaya |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | papaya latex enzyme | Carica papaya | - |
latex | - |
Carica papaya | - |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme can be activated to at least 65% of its original activity after storage at -80°C for up to 3 months | Carica papaya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxybenzoyl-Phe-Arg-7-(4-methyl)coumarylamide + H2O | fluorogenic substrate | Carica papaya | carboxybenzoyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | papain is the founding member of the large C1 family of papain-like cysteine proteases | Carica papaya | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
papain | - |
Carica papaya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Carica papaya |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000036 | - |
chagasin | inactivation of papain is very fast, rate constant for inactivation is 1500 mM/s | Carica papaya |