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Literature summary for 3.4.22.1 extracted from

  • Lee, J.; Kim, J.H.; Sohn, H.J.; Yang, H.J.; Na, B.K.; Chwae, Y.J.; Park, S.; Kim, K.; Shin, H.J.
    Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory-secretory proteins and their biochemical properties (2014), Parasitol. Res., 113, 2765-2776.
    View publication on PubMed
Search for more literature for 3.4.22.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Naegleria fowleri

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Naegleria fowleri
E-64d
-
 Naegleria fowleri
IAA
-
 Naegleria fowleri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0287
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
0.0358
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
0.0608
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
0.0656
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 calculated from cDNA Naegleria fowleri
38400
-
 calculated from cDNA Naegleria fowleri

Organism

Organism UniProt Comment Textmining
Naegleria fowleri X5D761
-
-
Naegleria fowleri X5D911
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity chromatography Naegleria fowleri

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression is detected in the trophozoite stage but not in the cyst stage Naegleria fowleri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Albumin + H2O
-
 Naegleria fowleri ?
-
 ?
benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Naegleria fowleri ?
-
 ?
benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide + H2O
-
 Naegleria fowleri ?
-
 ?
Collagen + H2O
-
 Naegleria fowleri ?
-
 ?
Fibronectin + H2O
-
 Naegleria fowleri ?
-
 ?
Hemoglobin + H2O
-
 Naegleria fowleri ?
-
 ?
IgA + H2O
-
 Naegleria fowleri ?
-
 ?
IgG + H2O
-
 Naegleria fowleri ?
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin B
-
 Naegleria fowleri
nfcpb
-
 Naegleria fowleri
nfcpb-L
-
 Naegleria fowleri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.318
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
0.327
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
0.476
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
0.512
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Naegleria fowleri

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5
-
 Naegleria fowleri

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8
-
 Naegleria fowleri
4 7
-
 Naegleria fowleri

Expression

Organism Comment Expression
Naegleria fowleri expression is detected in the trophozoite stage but not in the cyst stage up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.82
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
5.38
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
13.31
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
17.86
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri