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Literature summary for 3.4.22.1 extracted from

  • Kim, C.J.; Lee, D.I.; Zhang, D.; Lee, C.H.; Ahn, I.S.
    New strategy for selective and sensitive assay of cathepsin B using a dityrosine-based material (2013), Anal. Biochem., 435, 166-173.
    View publication on PubMed
Search for more literature for 3.4.22.1

Application

Application Comment Organism
molecular biology N,N'-diBoc-dityrosine-Gly-(isoniazid)2 is a sensitive and selective assay of cathepsin B activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00201
-
 N,N'-diBoc-dityrosine-Lys-(isoniazid)2 pH 6.2, 25°C Homo sapiens
0.00288
-
 N,N'-diBoc-dityrosine-Gly-(isoniazid)2 pH 6.2, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07858
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N'-diBoc-dityrosine-Gly-(isoniazid)2 + H2O
-
 Homo sapiens ?
-
 ?
N,N'-diBoc-dityrosine-Lys-(isoniazid)2 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin B
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
 N,N'-diBoc-dityrosine-Gly-(isoniazid)2 pH 6.2, 25°C Homo sapiens
0.036
-
 N,N'-diBoc-dityrosine-Lys-(isoniazid)2 pH 6.2, 25°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 N,N'-diBoc-dityrosine-Lys-(isoniazid)2 pH 6.2, 25°C Homo sapiens
3.87
-
 N,N'-diBoc-dityrosine-Gly-(isoniazid)2 pH 6.2, 25°C Homo sapiens