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Literature summary for 3.4.22.1 extracted from
- Cathepsin B of Cynoglossus semilaevis: identification, expression, and activity analysis (2012), Comp. Biochem. Physiol. B, 161, 54-59.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Cynoglossus semilaevis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H277E | the mutant is dramatically reduced in proteolytic activity. | Cynoglossus semilaevis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36250 | - |
x * 36250, calculated from amino acid sequence | Cynoglossus semilaevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cynoglossus semilaevis | G9FTP5 | tongue sole | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Cynoglossus semilaevis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cynoglossus semilaevis | - |
| gill | - |
Cynoglossus semilaevis | - |
| gut | - |
Cynoglossus semilaevis | - |
| head kidney | - |
Cynoglossus semilaevis | - |
| heart | lowest expression in heart | Cynoglossus semilaevis | - |
| liver | highest expression in liver | Cynoglossus semilaevis | - |
| muscle | - |
Cynoglossus semilaevis | - |
| spleen | - |
Cynoglossus semilaevis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Arg-L-Arg-4-nitroanilide + H2O | - |
Cynoglossus semilaevis | L-Arg-L-Arg + 4-nitroaniline | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36250, calculated from amino acid sequence | Cynoglossus semilaevis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CatB | - |
Cynoglossus semilaevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Cynoglossus semilaevis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | more than 60% of maximum activity between 20 und 50°C | Cynoglossus semilaevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Cynoglossus semilaevis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | more than 60% of maximum activity in the pH range of 5.0-6.5 | Cynoglossus semilaevis |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cynoglossus semilaevis | following experimental infection with Vibrio anguillarum, cathepsin B expression in head kidney increases to significant levels at 1 h, 4 h, 12 h, 24 h, and 48 h, with maximum induction (25fold) occurring at 4 h post-infection. In liver, significant inductions are observed at 1 h to 48 h post-infection, with maximum induction (36fold) occurring at 4 h post-infection | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | cathepsin B is involved in host immune response during bacterial infection and vaccination | Cynoglossus semilaevis |
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