Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activator buffer contains 5 mM EDTA, 10 mM DTT, pH 5.2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
caveola | of the lysosomal fraction, co-localization of active cathepsin B and its cell surface binding partners, S100A/p11 of the annexin II heterotetramer, to caveolae of human umbilical vein endothelial cells and colorectal carcinoma cells | Homo sapiens | 5901 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, single chain cathepsin B, SDS-PAGE, x * 25000-26000, heavy chain of double chain cathepsin B, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen IV + H2O | Homo sapiens | - |
? | - |
? | |
Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
additional information | Homo sapiens | the enzyme is involved in extracellular matrix degradation in caveolae of endothelial cells during tube formation, overview. Cathepsin B regulates both pro- and anti-angiogenic factors and may be a contributor to the angiogenic switch of endothelial cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially from caveolae of human umbilical vein endothelial cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic cancer cell | - |
Homo sapiens | - |
colorectal cancer cell | - |
Homo sapiens | - |
endothelial cell | umbilical vein endothelial cells, caveolae | Homo sapiens | - |
HCT-116 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen IV + H2O | - |
Homo sapiens | ? | - |
? | |
Collagen IV + H2O | quenched fluorescent protein | Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
Gelatin + H2O | quenched fluorescent protein | Homo sapiens | ? | - |
? | |
additional information | the enzyme is involved in extracellular matrix degradation in caveolae of endothelial cells during tube formation, overview. Cathepsin B regulates both pro- and anti-angiogenic factors and may be a contributor to the angiogenic switch of endothelial cells | Homo sapiens | ? | - |
? | |
N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine-4-methyl-7-coumarylamide + H2O | - |
Homo sapiens | N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine + 4-methyl-7-coumarylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, single chain cathepsin B, SDS-PAGE, x * 25000-26000, heavy chain of double chain cathepsin B, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cysteine cathepsin | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Homo sapiens |