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Literature summary for 3.4.22.1 extracted from

  • Koo, Y.D.; Ahn, J.E.; Salzman, R.A.; Moon, J.; Chi, Y.H.; Yun, D.J.; Lee, S.Y.; Koiwa, H.; Zhu-Salzman, K.
    Functional expression of an insect cathepsin B-like counter-defence protein (2008), Insect Mol. Biol., 17, 235-245.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information soyacystatin N induces expression of CmCatB1, but not of CmCatB2 Callosobruchus maculatus

Cloned(Commentary)

Cloned (Comment) Organism
gene CmCatB1, DNA and amino acid sequence determination and analysis, the native signal peptide of CmCatB1 is replaced by a yeast alpha-factor secretion signal sequence, the yeast peptide is subsequently cleaved and the recombinant C-terminally His6-tagged CmCatB1 proprotein is secreted to the cell culture medium of the transformed Pichia pastoris cells Callosobruchus maculatus
gene CmCatB2, DNA and amino acid sequence determination and analysis, CmCatB2 may be an inactive protease isoform resulting from splicing errors Callosobruchus maculatus

Inhibitors

Inhibitors Comment Organism Structure
CA-074 a cathepsin B-specific inhibitor, inhibition CmCatB1 Callosobruchus maculatus
E-64 a cysteine protease inhibitor, inhibition of CmCatB1 Callosobruchus maculatus
additional information no inhibition of CmCatB1 by PMSF, leupeptin, pepstatin A, ethanol and DMSO Callosobruchus maculatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Callosobruchus maculatus CmCatB2 may be an inactive protease isoform resulting from splicing errors ?
-
?

Organism

Organism UniProt Comment Textmining
Callosobruchus maculatus B2XVS9 CmCatB2; gene CmCatB2 probably resulting from alternate splicing
-
Callosobruchus maculatus Q5VJM8 CmCatB1; genes CmCatB1 and CmCatB2 probably resulting from alternate splicing
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylation of recombinant CmCatB1 by N-glycosidase F Callosobruchus maculatus

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged CmCatB1 proprotein from Pichia pastoris cell culture medium by nickel affinity chromatography Callosobruchus maculatus

Source Tissue

Source Tissue Comment Organism Textmining
gut larval Callosobruchus maculatus
-
larva
-
Callosobruchus maculatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
about 180 U, purified recombinant CmCatB1, 1 U is the amount of mature CmCatB1 required to produce an absorbance change of 0.01 optical density per h in a 1-cm cuvette, pH 4.5, 37°C Callosobruchus maculatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CmCatB2 may be an inactive protease isoform resulting from splicing errors Callosobruchus maculatus ?
-
?
N-benzoyl-Arg-Arg-4-nitroanilide + H2O
-
Callosobruchus maculatus N-benzoyl-Arg-Arg + 4-nitroaniline
-
?
protein rGSII-D88N + H2O
-
Callosobruchus maculatus ?
-
?

Synonyms

Synonyms Comment Organism
CatB1
-
Callosobruchus maculatus
cathepsin B-like counter-defence protein
-
Callosobruchus maculatus
cathepsin B-like cysteine protease 1
-
Callosobruchus maculatus
CmCatB1
-
Callosobruchus maculatus
CmCatB2
-
Callosobruchus maculatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Callosobruchus maculatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
CmCatB1 Callosobruchus maculatus

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 very low activity of CmCatB1 at pH 6.5 Callosobruchus maculatus