Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | soyacystatin N induces expression of CmCatB1, but not of CmCatB2 | Callosobruchus maculatus |
Cloned (Comment) | Organism |
---|---|
gene CmCatB1, DNA and amino acid sequence determination and analysis, the native signal peptide of CmCatB1 is replaced by a yeast alpha-factor secretion signal sequence, the yeast peptide is subsequently cleaved and the recombinant C-terminally His6-tagged CmCatB1 proprotein is secreted to the cell culture medium of the transformed Pichia pastoris cells | Callosobruchus maculatus |
gene CmCatB2, DNA and amino acid sequence determination and analysis, CmCatB2 may be an inactive protease isoform resulting from splicing errors | Callosobruchus maculatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CA-074 | a cathepsin B-specific inhibitor, inhibition CmCatB1 | Callosobruchus maculatus | |
E-64 | a cysteine protease inhibitor, inhibition of CmCatB1 | Callosobruchus maculatus | |
additional information | no inhibition of CmCatB1 by PMSF, leupeptin, pepstatin A, ethanol and DMSO | Callosobruchus maculatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Callosobruchus maculatus | CmCatB2 may be an inactive protease isoform resulting from splicing errors | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Callosobruchus maculatus | B2XVS9 | CmCatB2; gene CmCatB2 probably resulting from alternate splicing | - |
Callosobruchus maculatus | Q5VJM8 | CmCatB1; genes CmCatB1 and CmCatB2 probably resulting from alternate splicing | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation of recombinant CmCatB1 by N-glycosidase F | Callosobruchus maculatus |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged CmCatB1 proprotein from Pichia pastoris cell culture medium by nickel affinity chromatography | Callosobruchus maculatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | larval | Callosobruchus maculatus | - |
larva | - |
Callosobruchus maculatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
about 180 U, purified recombinant CmCatB1, 1 U is the amount of mature CmCatB1 required to produce an absorbance change of 0.01 optical density per h in a 1-cm cuvette, pH 4.5, 37°C | Callosobruchus maculatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CmCatB2 may be an inactive protease isoform resulting from splicing errors | Callosobruchus maculatus | ? | - |
? | |
N-benzoyl-Arg-Arg-4-nitroanilide + H2O | - |
Callosobruchus maculatus | N-benzoyl-Arg-Arg + 4-nitroaniline | - |
? | |
protein rGSII-D88N + H2O | - |
Callosobruchus maculatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CatB1 | - |
Callosobruchus maculatus |
cathepsin B-like counter-defence protein | - |
Callosobruchus maculatus |
cathepsin B-like cysteine protease 1 | - |
Callosobruchus maculatus |
CmCatB1 | - |
Callosobruchus maculatus |
CmCatB2 | - |
Callosobruchus maculatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Callosobruchus maculatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
CmCatB1 | Callosobruchus maculatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | very low activity of CmCatB1 at pH 6.5 | Callosobruchus maculatus |