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Literature summary for 3.4.22.1 extracted from

  • Sharma, S.; Mittal, A.; Gupta, V.K.; Singh, H.
    Improved stabilization of microencapsulated cathepsin B in harsh conditions (2007), Enzyme Microb. Technol., 40, 337-342.
No PubMed abstract available

General Stability

General Stability Organism
CM-cellulose and 1% CaCl2 concentration for cationic solution and 3% sodium alginate concentration for anionic solution, the encapsulated enzyme can retain about 60-70% of the immobilized activity even after five batch reactions whereas at other concentrations of CM-cellulose (2.0-3.0%), CaCl2 (1.5-2.5%) and sodium alginate (1.0-2.0%), the retention of enzyme activity is less (about 28-50%) Capra aegagrus
the encapsulated form of enzyme can retain about 60% of its original activity during its reusability for more than five successive batch reactions, the percent-encapsulated activity is about 53% at 4% CM-cellulose concentration, at 4% Capra aegagrus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.02
-
alpha-N-benzoyl-DL-Arg-beta-naphthylamide free enzyme Capra aegagrus
1.5 2 alpha-N-benzoyl-DL-Arg-beta-naphthylamide encapsulated enzyme Capra aegagrus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol at 15% (v/v) ethanol immobilized enzyme loses about 40% of its original activity whereas the free form loses almost all the enzyme activity Capra aegagrus

Organism

Organism UniProt Comment Textmining
Capra aegagrus
-
Capra aegagrus hircus
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Sephadex G-100 gel filtration, organomercurial affinity chromatography, and CM-Sephadex C-50 gel filtration Capra aegagrus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Capra aegagrus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-benzoyl-DL-Arg-beta-naphthylamide + H2O
-
Capra aegagrus alpha-N-benzoyl-DL-Arg + beta-naphthylamine
-
?

Synonyms

Synonyms Comment Organism
CatB
-
Capra aegagrus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
free enzyme Capra aegagrus
55
-
encapsulated enzyme Capra aegagrus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the free enzyme has a half-life of 141.7 min at 50°C and 12.9 min at 70°C, the encapsulated enzyme has a half-life of 1559.2 min at 50°C and 20.8 min at 70°C, the free enzyme loses almost whole of its activity when treated at 60°C for 45 min while the immobilized retains about 25% of its original activity Capra aegagrus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 encapsulated enzyme Capra aegagrus
6
-
free enzyme Capra aegagrus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5 encapsulated enzyme Capra aegagrus
4 6.5 free enzyme Capra aegagrus