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Literature summary for 3.4.22.1 extracted from

  • Premzl, A.; Turk, V.; Kos, J.
    Intracellular proteolytic activity of cathepsin B is associated with capillary-like tube formation by endothelial cells in vitro (2006), J. Cell. Biochem., 97, 1230-1240.
    View publication on PubMed
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
pepsin A
-
 Homo sapiens

Application

Application Comment Organism
drug development cathepsin B contributes to the neovascularization process and should be considered as a potential therapeutic target Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CA-074 Me 99.4% inhibition at 0.010 mM in cell lysates Homo sapiens
N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-L-isoleucyl-L-proline CA-074, 40.4% inhibition at 0.010 mM in cell lysates Homo sapiens
trans-epoxysuccinyl-L-leucyl-amido(4-guanidino)butane E-64, 63.2% inhibition at 0.010 mM in cell lysates Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-
lysosome
-
 Homo sapiens 5764
-
additional information Alexa Fluor 488 labeled intracellular cathepsin B is localized in vesicle-like structures in the perinuclear region of HUVECs Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P07858
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HUVEC cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
 secreted protein in cell culture, in the presence of 0.01 mM E-64 Homo sapiens
0.00042
-
 secreted protein in cell culture, in the presence of 0.01 mM CA-074 Homo sapiens
0.00042
-
 secreted protein in cell culture, in the presence of 0.01 mM CA-074 Me Homo sapiens
0.00225
-
 in the presence of 0.01 mM CA-074 Me in cell lysates Homo sapiens
0.1027
-
 in the presence of 0.01 mM E-64 in cell lysates Homo sapiens
0.1468
-
 in the presence of 0.01 mM CA-074 in cell lysates Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Arg-Arg-cresyl violet + H2O
-
 Homo sapiens Z-Arg-Arg + cresyl violet
-
 ?
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O
-
 Homo sapiens Z-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin B
-
 Homo sapiens