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Literature summary for 3.4.22.1 extracted from

  • Dvorak, J.; Delcroix, M.; Rossi, A.; Vopalensky, V.; Pospisek, M.; Sedinova, M.; Mikes, L.; Sajid, M.; Sali, A.; McKerrow, J.H.; Horak, P.; Caffrey, C.R.
    Multiple cathepsin B isoforms in schistosomula of Trichobilharzia regenti: identification, characterisation and putative role in migration and nutrition (2005), Int. J. Parasitol., 35, 895-910.
    View publication on PubMed
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
asparaginyl endopeptidase from Schistosoma mansoni
-
 Trichobilharzia regenti

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trichobilharzia regenti
expression in Escherichia coli, expressed as zymogen in Pichia pastoris Trichobilharzia regenti

Inhibitors

Inhibitors Comment Organism Structure
DCG-04
-
 Trichobilharzia regenti
N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-L-isoleucyl-L-proline CA-074; CA-074; CA-074; CA-074 Trichobilharzia regenti
trans-epoxysuccinyl-L-leucyl-amido(4-guanidino)butane E-64; E-64; E-64 Trichobilharzia regenti
trans-epoxysuccinyl-L-leucyl-smido(4-guanidino)butane E-64 Trichobilharzia regenti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
 mature protein, calculated from amino acid sequence Trichobilharzia regenti
33000
-
 x * 33000, SDS-PAGE Trichobilharzia regenti
36500
-
 zymogen protein, calculated from amino acid sequence Trichobilharzia regenti
38700
-
 full length protein, calculated from amino acid sequence Trichobilharzia regenti

Organism

Organism UniProt Comment Textmining
Trichobilharzia regenti Q4VRW4
-
-
Trichobilharzia regenti Q4VRW5
-
-
Trichobilharzia regenti Q4VRW6
-
-
Trichobilharzia regenti Q4VRW7
-
-
Trichobilharzia regenti Q4VRW8
-
-
Trichobilharzia regenti Q4VRW9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichobilharzia regenti

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-phenylalanyl-arginine-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, activity at pH 5.0 is 80fold greater than with Arg-Arg Trichobilharzia regenti Z-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information no degradation of hemoglobin Trichobilharzia regenti ?
-
 ?
additional information this isoform encodes an inactive enzyme Trichobilharzia regenti ?
-
 ?
myelin basic protein + H2O
-
 Trichobilharzia regenti ?
-
 ?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, least preferred substrate Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, negligible hydrolysis Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Leu-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature Trichobilharzia regenti Z-Arg-Leu + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Leu-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, less than 25% hydrolysis compared to Z-Arg-Val-7-amido-4-methylcoumarin Trichobilharzia regenti Z-Arg-Leu + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Phe-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature Trichobilharzia regenti Z-Arg-Phe + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Phe-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, less than 25% hydrolysis compared to Z-Arg-Val-7-amido-4-methylcoumarin Trichobilharzia regenti Z-Arg-Phe + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-Val-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, preferred substrate Trichobilharzia regenti Z-Arg-Val + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Trichobilharzia regenti

Synonyms

Synonyms Comment Organism
cathepsin B isoform 1 Trichobilharzia regenti
cathepsin B isoform 2 Trichobilharzia regenti
cathepsin B isoform 3 Trichobilharzia regenti
cathepsin B isoform 4 Trichobilharzia regenti
cathepsin B isoform 5 Trichobilharzia regenti
cathepsin B isoform 6 Trichobilharzia regenti
TrCB1.1
-
 Trichobilharzia regenti
TrCB1.2
-
 Trichobilharzia regenti
TrCB1.3
-
 Trichobilharzia regenti
TrCB1.4
-
 Trichobilharzia regenti
TrCB1.5
-
 Trichobilharzia regenti
TrCB1.6
-
 Trichobilharzia regenti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6.5 with Z-Arg-Phe-7-amido-4-methylcoumarin, Z-Arg-Val-7-amido-4-methylcoumarin or Z-Arg-Leu-7-amido-4-methylcoumarin as substrate Trichobilharzia regenti
4.5 5.5 with Z-Phe-Arg-7-amido-4-methylcoumarin as substrate Trichobilharzia regenti
5 6.5 with Z-Arg-Val-7-amido-4-methylcoumarin as substrate Trichobilharzia regenti
5.5 6.5 with Z-Arg-Arg-7-amido-4-methylcoumarin as substrate Trichobilharzia regenti

pH Range

pH Minimum pH Maximum Comment Organism
4 7 when myelin basic protein is used as substrate Trichobilharzia regenti