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Literature summary for 3.4.22.1 extracted from

  • Kawada, A.; Hara, K.; Morimoto, K.; Hiruma, M.; Ishibashi, A.
    Rat epidermal cathepsin B: purification and characterization of proteolytic properties toward filaggrin and synthetic substrates (1995), Int. J. Biochem. Cell Biol., 27, 175-183.
    View publication on PubMed
Search for more literature for 3.4.22.1

Inhibitors

Inhibitors Comment Organism Structure
chymostatin
-
 Rattus norvegicus
L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Rattus norvegicus
leupeptin
-
 Rattus norvegicus
N-tosyl-lysyl chloromethylketone
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
skin epidermis Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.152
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
0.424
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
-
 Rattus norvegicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
filaggrin + H2O
-
 Rattus norvegicus hydrolyzed filaggrin
-
 ?
Proteins + H2O
-
 Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 stable for 30 min Rattus norvegicus
30
-
 stable for 30 min Rattus norvegicus
50
-
 40% activity remaining after 30 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide as substrate, filaggrin is hydrolyzed at pH 4.0, but not at pH 7.0 Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6.5 stable Rattus norvegicus