Protein Variants | Comment | Organism |
---|---|---|
R504Q | site-directed mutagenesis, construction of a MASP-1 catalytic fragment, that has a molecular weight of 45.5 kDa and consists of the three C-terminal domains complement control protein 1 and 2 followed by the serine protease domain | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C2 + H2O | Homo sapiens | - |
? | - |
? | |
fibrinogen + H2O | Homo sapiens | MASP-1-induced fibrin formation and activation is thrombin-dependent, in plasma environment fibrin formation is not directly induced by MASP-1 in the absence of prothrombin | fibrin + ? | - |
? | |
proform factor XIII + H2O | Homo sapiens | activation | mature factor XIII + ? | - |
? | |
proform thrombin-activatable fibrinolysis inhibitor + H2O | Homo sapiens | activation | mature thrombin-activatable fibrinolysis inhibitor + ? | - |
? | |
prothrombin + H2O | Homo sapiens | activation | thrombin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P48740 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C2 + H2O | - |
Homo sapiens | ? | - |
? | |
fibrinogen + H2O | low activity | Homo sapiens | fibrin + ? | - |
? | |
fibrinogen + H2O | MASP-1-induced fibrin formation and activation is thrombin-dependent, in plasma environment fibrin formation is not directly induced by MASP-1 in the absence of prothrombin | Homo sapiens | fibrin + ? | - |
? | |
additional information | no activity with complement component C4. Direct activation of C3 convertase by MASP-1 occurs with very low catalytic efficiency and is not of physiological relevance | Homo sapiens | ? | - |
? | |
proform factor XIII + H2O | activation | Homo sapiens | mature factor XIII + ? | - |
? | |
proform factor XIII + H2O | the Val34 variant is a better substrate than the Leu34 variant | Homo sapiens | mature factor XIII + ? | - |
? | |
proform thrombin-activatable fibrinolysis inhibitor + H2O | - |
Homo sapiens | mature thrombin-activatable fibrinolysis inhibitor + ? | - |
? | |
proform thrombin-activatable fibrinolysis inhibitor + H2O | activation | Homo sapiens | mature thrombin-activatable fibrinolysis inhibitor + ? | - |
? | |
prothrombin + H2O | - |
Homo sapiens | thrombin + ? | - |
? | |
prothrombin + H2O | activation | Homo sapiens | thrombin + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MASP-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
mutant R504Q catalytic fragment, the mutant is resistant to autolysis and preserves its enzymatic activity during prolonged incubation, estimated half-life of several hours in plasma at 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | MASP-1 cleaves complement component C2 but not complement component C4 and therefore is not capable of generating C3 convertase alone | Homo sapiens |