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Literature summary for 3.4.21.B7 extracted from

  • Ambrus, G.; Gal, P.; Kojima, M.; Szilagyi, K.; Balczer, J.; Antal, J.; Graf, L.; Laich, A.; Moffatt, B.E.; Schwaeble, W.; Sim, R.B.; Zavodszky, P.
    Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments (2003), J. Immunol., 170, 1374-1382.
    View publication on PubMed
Search for more literature for 3.4.21.B7

Cloned(Commentary)

Cloned (Comment) Organism
catalytic region of MASP-1 and MASP-2, consisting of the two CCP modules and the SP domain (CCP1-CCP2-SP) is expressed in Escherichia coli BL-21 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin may be a physiological inhibitor, 1-3fold molar excess, forms a stable complex with the enzyme Homo sapiens
C1-inhibitor completely blocks enzyme activity, 1-3fold molar excess, forms a stable complex with the enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
 Complement component C2 37°C, cleavage by the MASP-1 CCP1-CCP2-SP fragment Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P48740
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C2 + H2O digested by the MASP-1 CCP1-CCP2-SP fragment at a moderate rate Homo sapiens ?
-
 ?
complement component C3i + H2O cleavage of intact C3i (C3 with a reacted thiolester bond) by the MASP-1 CCP1-CCP2-SP fragment with a low but significant efficiency Homo sapiens ?
-
 ?
complement-activating component of Ra-reactive factor + H2O cleavage at Arg448-/-Ile449 Homo sapiens ?
-
 ?
MASP1 mannan-binding lectin serine protease 1 isoform 1 precursor + H2O autodegradation pattern of the MASP-1 CCP1-CCP2-SP fragment. Cleavage occurs at the Arg504-Asp505 bond, which results in the removal of a 6000 Da fragment from the active enzyme. The autolysis of the MASP-1 CCP1-CCP2-SP fragment causes the loss of its enzymatic activity due to the removal of the histidine from the catalytic triad Homo sapiens ?
-
 ?
additional information complement component C4, similar to C3, is basically resistant to the proteolytic activity of MASP-1. MASP-1 shows extreme Arg selectivity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
mannan-binding lectin-associated serine protease-1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Complement component C2 37°C, cleavage by the MASP-1 CCP1-CCP2-SP fragment Homo sapiens