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Literature summary for 3.4.21.B6 extracted from

  • Buzza, M.S.; Martin, E.W.; Driesbaugh, K.H.; Desilets, A.; Leduc, R.; Antalis, T.M.
    Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway (2013), J. Biol. Chem., 288, 10328-10337.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens activated prostasin appears to target several downstream effector proteins, including the epithelial sodium channel and the G protein-coupled protease activated receptor-2, which are both matriptase substrates ?
-
 ?
proform epithelial sodium channel + H2O Homo sapiens
-
 mature epithelial sodium channel + ?
-
 ?
proform G protein-coupled protease activated receptor-2 + H2O Homo sapiens
-
 mature G protein-coupled protease activated receptor-2 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16651
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification matriptase is an effective activator of the prostasin zymogen through direct proteolytic cleavage at its zymogen activation site. Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell
-
 Homo sapiens
-
epidermis
-
 Homo sapiens
-
epithelium
-
 Homo sapiens
-
intestine
-
 Homo sapiens
-
additional information prostasin is co-expressed with matriptase and increases during human Caco-2 cell differentiation and barrier formation Homo sapiens
-
skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information activated prostasin appears to target several downstream effector proteins, including the epithelial sodium channel and the G protein-coupled protease activated receptor-2, which are both matriptase substrates Homo sapiens ?
-
 ?
additional information the matriptase zymogen is no substrate of prostasin Homo sapiens ?
-
 ?
proform epithelial sodium channel + H2O
-
 Homo sapiens mature epithelial sodium channel + ?
-
 ?
proform G protein-coupled protease activated receptor-2 + H2O
-
 Homo sapiens mature G protein-coupled protease activated receptor-2 + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Homo sapiens

Expression

Organism Comment Expression
Homo sapiens prostasin expression increases during human Caco-2 cell differentiation and barrier formation up

General Information

General Information Comment Organism
metabolism matriptase and not prostasin is the primary effector protease of tight junction assembly in simple columnar epithelia Homo sapiens
physiological function Prostasin catalyzed activation of ENaC induces an open channel conformation associated with sodium influx that results in membrane depolarization. Prostasin together with matriptase comprise a single common proteolytic pathway,that is required for barrier formation. Prostasin is activated through proteolytic cleavage by matriptase, but prostasin is also required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway Homo sapiens