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Literature summary for 3.4.21.B6 extracted from

  • Rickert, K.W.; Kelley, P.; Byrne, N.J.; Diehl, R.E.; Hall, D.L.; Montalvo, A.M.; Reid, J.C.; Shipman, J.M.; Thomas, B.W.; Munshi, S.K.; Darke, P.L.; Su, H.P.
    Structure of human prostasin, a target for the regulation of hypertension (2008), J. Biol. Chem., 283, 34864-34872.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B6

Cloned(Commentary)

Cloned (Comment) Organism
expression of the prostasin proenzyme in Escherichia coli as insoluble inclusion bodies, refolding and activating via proteolytic removal of the N-terminal propeptide Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method. Active prostasin is crystallized, and the structure is determined to 1.45 A resolution. These apoprotein crystals are soaked with nafamostat, allowing the structure of the inhibited acyl-enzyme intermediate structure to be determined to 2.0 A resolution Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0335
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 801 expressed in Sf21 cells, glycosylated Homo sapiens
0.0343
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 28 expressed in Escherichia coli, not glycosylated Homo sapiens
0.0388
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 26 expressed in Escherichia coli, not glycosylated Homo sapiens
0.04
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 40 expressed in Sf21 cells, not glycosylated Homo sapiens
0.043
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 35 expressed in Sf21 cells, glycosylated Homo sapiens
0.0493
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 1 expressed in mouse cells, glycosylated Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 purified refolded prostasin, gel filtration Homo sapiens
27879
-
 1 * 27879, refolded and purified prostasin variant 26, calculated from sequence Homo sapiens
28351
-
 1 * 28351, refolded and purified prostasin variant 28, calculated from sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16651
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-KHYR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens acetyl-KHYR + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27879, refolded and purified prostasin variant 26, calculated from sequence Homo sapiens
monomer 1 * 28351, refolded and purified prostasin variant 28, calculated from sequence Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 26 expressed in Escherichia coli, not glycosylated Homo sapiens
0.13
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 40 expressed in Sf21 cells, not glycosylated Homo sapiens
0.14
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 28 expressed in Escherichia coli, not glycosylated Homo sapiens
0.17
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 35 expressed in Sf21 cells, glycosylated Homo sapiens
0.23
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 801 expressed in Sf21 cells, glycosylated Homo sapiens
0.27
-
 acetyl-KHYR-7-amido-4-methylcoumarin pH 9, 23°C, variant 1 expressed in mouse cells, glycosylated Homo sapiens