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Literature summary for 3.4.21.B6 extracted from
- Prostasin, a membrane-anchored serine peptidase, regulates sodium currents in JME/CF15 cells, a cystic fibrosis airway epithelial cell line (2004), Am. J. Physiol. Lung Cell Mol. Physiol., 287, L928-L935.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | is a major regulator of epithelial Na+ channel-mediated Na+ current, airway prostasin is a target for therapeutic inhibition to normalize ion current in cystic fibrosis airway | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pCRT7/NT-TOPO and expressed in Escherichia coli BL21(DE3)pLysS, into pcDNA3.1/V5-His-TOPO and expressed in CHO cells and HEK-293 cells, overexpression in CF epithelial cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | prostasin gene silencing, drops epithelial Na+ channel currents to 26% of baseline | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aprotinin | completely inhibits | Homo sapiens |  |
| benzamidine | completely inhibits | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
immunoblotting | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by sequential anion exchange and aprotinin affinity chromatography, by Ni-NTA His-bind chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | - |
Homo sapiens | - |
| Calu-3 cell | - |
Homo sapiens | - |
| additional information | natively expressed in JME/CF15 cells, cystic fibrosis airway epithelial cell line, little if any detectable in CHO cells and HEK-293 cells | Homo sapiens | - |
| semen | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tosyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
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