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Literature summary for 3.4.21.B6 extracted from

  • Yu, J.X.; Chao, L.; Chao, J.
    Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue distribution, and localization in prostate gland (1994), J. Biol. Chem., 269, 18843-18848.
    View publication on PubMed
Search for more literature for 3.4.21.B6

Inhibitors

Inhibitors Comment Organism Structure
antipain 50% inhibition at 0.0064 mM Homo sapiens
Aprotinin 50% inhibition at 0.0000018 mM Homo sapiens
benzamidine 50% inhibition at 0.86 mM Homo sapiens
leupeptin 50% inhibition at 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
108
-
 D-Pro-Phe-Arg 7-amido-4-methylcoumarin pH 9, 25°C Homo sapiens
108
-
 D-Pro-Phe-Arg 7-amido-4-methylcoumarin D-Pro-Phe-Arg-7-amido-4-methylcoumarin Homo sapiens
255
-
 D-Val-Leu-Arg-7-amido-4-methylcoumarin pH 9, 25°C Homo sapiens
717
-
 Z-Gly-Pro-Arg-7-amido-4-trifluoromethylcoumarin pH 9, 25°C Homo sapiens
827
-
 D-Phe-Phe-Arg 7-amido-4-methylcoumarin pH 9, 25°C Homo sapiens
827
-
 D-Phe-Phe-Arg 7-amido-4-methylcoumarin D-Phe-Phe-Arg-7-amido-4-methylcoumarin Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
bronchus
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
additional information no activity in brain, muscle, testis, ventricle, atrium and aorta Homo sapiens
-
pancreas
-
 Homo sapiens
-
prostate gland epithelial cell and duct Homo sapiens
-
salivary gland
-
 Homo sapiens
-
seminal plasma
-
 Homo sapiens
-
urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-trifluoromethylcoumarin
-
 Homo sapiens benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-trifluoromethylcoumarin
-
 ?
D-Phe-Phe-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens ?
-
 ?
D-Phe-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens D-Phe-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
D-Pro-Phe-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens ?
-
 ?
D-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens D-Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
D-Val-Leu-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens ?
-
 ?
D-Val-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens D-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
Protein + H2O
-
 Homo sapiens ?
-
 ?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
S01.159 Merops ID Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.068
-
 D-Pro-Phe-Arg-7-amido-4-methylcoumarin pH 9, 25°C Homo sapiens
0.105
-
 D-Val-Leu-Arg-7-amido-4-methylcoumarin pH 9, 25°C Homo sapiens
0.287
-
 Z-Gly-Pro-Arg-7-amido-4-trifluoromethylcoumarin pH 9, 25°C Homo sapiens
0.29
-
 D-Phe-Phe-Arg-7-amido-4-methylcoumarin pH 9, 25°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5 50% activity at pH 8.5 and pH 9.5 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing, pH gradient 3.5-10
-
 4.5