Cloned (Comment) | Organism |
---|---|
expression of wid-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of IS1-deletion mutants of S324A and S324C enzyme variants | Thermococcus kodakarensis |
S324A | site-directed mutagenesis, structure comparison of the mutant pro-enzyme with the wild-type pro-enzyme | Thermococcus kodakarensis |
S324C | site-directed mutagenesis, structure comparison of the mutant pro-enzyme with the wild-type pro-enzyme | Thermococcus kodakarensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted into the external medium, probably as inactive pro-enzyme, with the assistance of the signal sequence | Thermococcus kodakarensis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermococcus kodakarensis | the propeptide is effectively degraded by the mature enzyme only at high temperatures, because it is too stable to be degraded at moderate temperatures | ? | - |
? | |
pro-Tk-subtilisin + H2O | Thermococcus kodakarensis | autoactivation | Tk-subtilisin + propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | P58502 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | prepro-Tk-subtilisin (Prepro-TKS), which consists of the signal sequence [Met (-24)-Ala(-1)], propeptide (Gly1-Leu69), and mature domain (Tk-subtilisin, Gly70-Gly398). Tk-subtilisin matures from Pro-Tk-subtilisin upon autoprocessing and degradation of propeptide. The pro-enzyme form contains the insertion sequence, IS1, at the N-terminus of the mature domain which is required not only for hyperstabilization of Pro-Tk-subtilisin but also for its rapid maturation, Most part of IS1 (Gly70-Gly78) is autocatalytically removed when Pro-TKS matures to Tk-subtilisin, structure and mechanism, overview | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the propeptide is effectively degraded by the mature enzyme only at high temperatures, because it is too stable to be degraded at moderate temperatures | Thermococcus kodakarensis | ? | - |
? | |
pro-Tk-subtilisin + H2O | autoactivation | Thermococcus kodakarensis | Tk-subtilisin + propeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tk-subtilisin | - |
Thermococcus kodakarensis |
TKS | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Thermococcus kodakarensis |
General Information | Comment | Organism |
---|---|---|
additional information | prepro-Tk-subtilisin (Prepro-TKS), which consists of the signal sequence [Met (-24)-Ala(-1)], propeptide (Tkpro, Gly1-Leu69), and mature domain (Tk-subtilisin, Gly70-Gly398). the pro-enzyme form contains the insertion sequence, IS1, at the N-terminus of the mature domain which is required not only for hyperstabilization of Pro-TKS but also for its rapid maturation | Thermococcus kodakarensis |