Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 61% loss of activity in absence of CaCl2 | Aeropyrum pernix | |
dithiothreitol | 5 mM, 64% loss of activity in absence of CaCl2 | Aeropyrum pernix | |
EDTA | 5 mM, complete loss of activity | Aeropyrum pernix | |
EGTA | 5 mM, complete loss of activity | Aeropyrum pernix | |
Guanidine HCl | 5 mM, 65% loss of activity in absence of CaCl2, activation in presence of 1 mM CaCl2 | Aeropyrum pernix | |
PMSF | 5 mM, complete loss of activity | Aeropyrum pernix | |
SDS | 3%, 80% loss of activity in absence of CaCl2 | Aeropyrum pernix | |
Urea | 5 mM, 50% loss of activity in absence of CaCl2 | Aeropyrum pernix |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aeropyrum pernix | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | maximum enhancement of the purified pernisine activity is observed at around 1 mM CaCl2. Further increasing the CaCl2 above 1 mM leads to a gradual decline in this enhanced activity. In the presence of 1 mM CaCl2, the proteolytic activity of pernisine is enhanced, to reach around 75% greater activity, with the maximum activity at 105.0°C | Aeropyrum pernix | |
additional information | NaCl and CaCl2 together do not show any cumulative effects, as they appear to separately affect the activity | Aeropyrum pernix | |
NaCl | the maximal activity of the purified pernisine seen for 20 mM NaCl in the absence of 1 mM CaCl2 increases to more than 2fold after the addition of CaCl2, with even larger relative enhancement by CaCl2 at higher NaCl concentrations | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms | Aeropyrum pernix |
34000 | - |
x * 34000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Aeropyrum pernix DSM 11879 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the purified pernisine has a proregion that is autocleaved during maturation | Aeropyrum pernix |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
abnormal prion protein PrP(Sc) + H2O | the prion protein PrP(Sc) is a pathological prion protein PrP isoform. The proteolytic activity of pernisine does not depend on the species of origin of the PrP used (bovine, mouse, human) | Aeropyrum pernix | ? | - |
? | |
abnormal prion protein PrP(Sc) + H2O | the prion protein PrP(Sc) is a pathological prion protein PrP isoform. The proteolytic activity of pernisine does not depend on the species of origin of the PrP used (bovine, mouse, human) | Aeropyrum pernix DSM 11879 | ? | - |
? | |
azocasein + H2O | - |
Aeropyrum pernix | ? | - |
? | |
azocasein + H2O | - |
Aeropyrum pernix DSM 11879 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms | Aeropyrum pernix |
? | x * 34000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms | Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
substrate: azocasein, in absence of CaCl2 | Aeropyrum pernix |
105 | - |
substrate: azocasein, in the presence of 1 mM CaCl2 | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | 99 | active between 58 °C and 99 °C | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, stable in presence of CaCls, about 60% loss of activity in absence of CaCl2 | Aeropyrum pernix |
90 | - |
20 min, stable in presence of CaCl2, about 80% loss of activity in absence of CaCl2 | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
in the presence of 1 mM CaCl2, at 92°C | Aeropyrum pernix |
7 | - |
in absence of CaCl2, at 92°C | Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | active between pH 3.0 and pH 10.0, in the presence of 1 mM CaCl2, at 92°C | Aeropyrum pernix |
3.5 | 8 | active between pH 3.5 and pH 8.0, in absence of CaCl2, at 92°C | Aeropyrum pernix |