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Literature summary for 3.4.21.B57 extracted from

  • Hirata, A.; Hori, Y.; Koga, Y.; Okada, J.; Sakudo, A.; Ikuta, K.; Kanaya, S.; Takano, K.
    Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein (2013), BMC Biotechnol., 13, 19.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the activities in the presence of 1% SANISOL C and AMPHITOL 20 N are higher than those at the concentration of 0.1%. This may relate to the critical micelle concentration of the surfactants. Improvement of the enzyme activity in the presence of nonionic surfactants might be due to the stimulation of conformational changes in Tk-SP or the substrate, leading to activity gain Thermococcus kodakarensis

Application

Application Comment Organism
medicine potential application for the inactivation of abnormal prion protein PrPSc, a protease-resistant isoform of normal prion protein. PrPSc is largely unaffected by standard methods of sterilization, thus, contaminated neurosurgical instruments are the major cause of human transmissible spongiform encephalopathies Thermococcus kodakarensis
medicine the enzyme may have potential application as a detergent additive to decrease the infectivity of abnormal prion protein PrPSc Thermococcus kodakarensis
medicine Tk-SP-containing detergents can be developed to decrease the secondary infection risks of transmissible spongiform encephalopathies Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

General Stability

General Stability Organism
in the presence of 0.05% (w/v) nonionic surfactants, such as EMULGEN LS-114 or RHEODOL Tw-0120 V, and 0.01% (w/v) EDTA, Tk-SP retains almost its entire initial activity Thermococcus kodakarensis
in the presence of anionic surfactants, the enzyme is unstable, losing up to 80% of its activity Thermococcus kodakarensis
in the presence of anionic surfactants, Tk-SP is unstable, losing up to 80% of its activity Thermococcus kodakarensis
less stable in the presence of QUARTAMIN 60 W Thermococcus kodakarensis
stable in the presence of 0.1% (w/v) AMPHITOL 20Y-B Thermococcus kodakarensis
the enzyme is highly stable in the presence of 0.05% (w/v) nonionic surfactants and 0.01% (w/v) EDTA, retaining up to 80% of its activity Thermococcus kodakarensis
the enzyme is highly stable in the presence of both 0.1 and 1% (w/v) nonionic surfactants Thermococcus kodakarensis
the enzyme retains its activity in the presence of surfactants tested at 80°C and 90°C Thermococcus kodakarensis
the enzyme retains more than 100% of its activity in the presence of four of the nonionic surfactants, namely, EMULGEN 147, EMULGEN LS-114, EMULGEN PP-290, and RHEODOL Tw-0120 V. It is less stable in the presence of QUARTAMIN 60 W Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
AMPHITOL 20Y-B presence of 1% (w/v) AMPHITOL 20Y-B causes strong inhibitions, resulting in an enzyme retaining only 50% of its activity Thermococcus kodakarensis
EDTA about 20% loss of activity in the presence of 0.01% (w/v) at 80°C, about 60% loss of activity in the presence of 1% (w/v) at 80°C; activity decreases with the increasing concentration of EDTA from 0.01 to 1% (w/v) Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis P58502 sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106)
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
abnormal prion protein PrP(Sc) + H2O
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Thermococcus kodakarensis ?
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?
abnormal prion protein PrP(Sc) + H2O prion protein PrP(Sc) is a pathological prion protein PrP isoform. The enzyme can disrupt PrPSc to a level undetectable by Western-blot analysis Thermococcus kodakarensis ?
-
?
azocasein + H2O
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Thermococcus kodakarensis ?
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?

Synonyms

Synonyms Comment Organism
Tk-SP
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Thermococcus kodakarensis
Tk-subtilisin
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Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
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assay at Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Thermococcus kodakarensis