Cloned (Comment) | Organism |
---|---|
overproduction of pro-S255A in Escherichia coli BL21-Codon-Plus(DE3) | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
crystallization of the active-site mutant S255A of pro-Tk-subtilisin. The crystal is grown at 4°C by the sitting-drop vapour-diffusion method. Native X-ray diffraction data are collected to 2.3 A resolution.They crystal belongs to the orthorhombic space group I222, with unit-cell parameters a = 92.69, b = 121.78, c = 77.53 A. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient V(M) was calculated to be 2.6 A(3) Da(-1) and the solvent content was 53.1% | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
S255A | active-site mutant enzyme | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
active-site mutant S255A of pro-Tk-subtilisin | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | P58502 | sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106) | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, active-site mutant S255A of pro-Tk-subtilisin, SDS-PAGE | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
Tk-subtilisin | - |
Thermococcus kodakarensis |