Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | 0.5 mM, complete inhibition | Pyrococcus furiosus | |
EDTA | 10 mM, 27% inhibition | Pyrococcus furiosus | |
additional information | no inhibition by cysteine-type protease inhibitors pCMB and iodoacetate | Pyrococcus furiosus | |
PMSF | 1 mM, 87% inhibition | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell envelope | 75% of the activity is associated with the cell-envelope | Pyrococcus furiosus | 30313 | - |
cytoplasm | 25% of the activity was located in the cytoplasm | Pyrococcus furiosus | 5737 | - |
additional information | no activity is observed in the medium when Pyrococcus furiosus is grown in batch culture | Pyrococcus furiosus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P72186 | - |
- |
Oxidation Stability | Organism |
---|---|
oxygen-stable enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Pyrococcus furiosus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
115 | - |
- |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
t1/2 is above 6000 min | Pyrococcus furiosus |
95 | - |
t1/2: 540 min | Pyrococcus furiosus |
100 | - |
t1/2: 240 min | Pyrococcus furiosus |
105 | - |
t1/2: 20 min | Pyrococcus furiosus |
110 | - |
t1/2: 3 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | broad pH range with at least 50% of its maximal activity between pH 6.5 and 10.5 | Pyrococcus furiosus |