Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion technique. The best crystals are obtained when using 100 mM citrate (pH 5.5) and 17.5% polyethylene glycol 400 or 100 mM sodium acetate (pH 4.5), 50 mM CdCl2 and 28% polyethylene glycol 400 as reservoir solutions. Crystal structures of inactive 3-mers and active 12-mers of DegQ reveal molecular details of elements of a conserved allosteric activation cascade that defines distinct protease ON and OFF states | Legionella pneumophila subsp. pneumophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila subsp. pneumophila | Q5ZVV9 | - |
- |
Legionella pneumophila subsp. pneumophila ATCC 33152 | Q5ZVV9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DPMFKLV-4-methylcoumaryl-7-amide + H2O | - |
Legionella pneumophila subsp. pneumophila | ? | - |
? | |
DPMFKLV-4-methylcoumaryl-7-amide + H2O | - |
Legionella pneumophila subsp. pneumophila ATCC 33152 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | DegQLp forms predominantly trimers in the substrate-free state. In vitro, oligomerization is influenced by the pH of the protein solution and the presence of co-purified peptides binding to the PDZ1 and/or protease site. Misfolded proteins or peptides promote the assembly of protease active 12- or 24-mers the assembly of proteolytically active | Legionella pneumophila subsp. pneumophila |
Synonyms | Comment | Organism |
---|---|---|
DegQ | - |
Legionella pneumophila subsp. pneumophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Legionella pneumophila subsp. pneumophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Legionella pneumophila subsp. pneumophila |