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Literature summary for 3.4.21.B48 extracted from

  • Comellas-Bigler, M.; Maskos, K.; Huber, R.; Oyama, H.; Oda, K.; Bode, W.
    1.2 A Crystal structure of the serine carboxyl proteinase pro-kumamolisin: structure of an intact pro-subtilase (2004), Structure, 12, 1313-1323.
    View publication on PubMed
Search for more literature for 3.4.21.B48

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant E32A, W129A and S278A pro-kumamolisin are crystallized by sitting-drop vapor-diffusion method, 1.2/1.3 A crystal structure Bacillus novosp.

Protein Variants

Protein Variants Comment Organism
E32A the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme Bacillus novosp.
S278A the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitzo)Phe-Arg-Leu is reduced to 0.5% of the native enzyme Bacillus novosp.
W129A the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 3.8% of the native enzyme Bacillus novosp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, wild-type enzyme Bacillus novosp.
0.084
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme S278A Bacillus novosp.
0.089
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme W129A Bacillus novosp.
0.102
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme E32A Bacillus novosp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus novosp.
-
-

Organism

Organism UniProt Comment Textmining
Bacillus novosp.
-
 MN-32
-
Bacillus novosp. MN-32
-
 MN-32
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification pro-kumamolisinS278A: the prodomain exhibits a half-beta sandwich core docking to the catalytic domain similarly as the equivalent subtilisin prodomain complexes Bacillus novosp.

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus novosp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Bacillus novosp. Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu
-
 ?
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Bacillus novosp. MN-32 Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, wild-type enzyme Bacillus novosp.
0.08
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme S278A Bacillus novosp.
0.67
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme W129A Bacillus novosp.
1.24
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme E32A Bacillus novosp.
6.02
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, wild-type enzyme Bacillus novosp.
6.08
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme E32A Bacillus novosp.
6.08
-
 Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu pH 3.5, 60°C, mutant enzyme W129A Bacillus novosp.