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Literature summary for 3.4.21.B42 extracted from

  • Mitsui, S.; Yamada, T.; Okui, A.; Kominami, K.; Uemura, H.; Yamaguchi, N.
    A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate (2000), Biochem. Biophys. Res. Commun., 272, 205-211.
    View publication on PubMed
Search for more literature for 3.4.21.B42

Cloned(Commentary)

Cloned (Comment) Organism
gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection Homo sapiens
gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion into the medium Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Homo sapiens
44000
-
 x * 44000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBX7 prostate-type isozyme
-
Homo sapiens Q9UBX7 splicing variant 1
-
Homo sapiens Q9UBX7 brain-type isozyme
-
Homo sapiens Q9UBX7 splicing variant 2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 4 possible glycosylation sites Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of polypeptides, preferential cleavage site: Arg- enzyme is trypsin-like a serine protease Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
brain brain-type isozyme, secreted into the medium Homo sapiens
-
epithelium prostate Homo sapiens
-
heart brain-type isozyme Homo sapiens
-
hippocampus especially expressed in pyramidal neurons Homo sapiens
-
lung prostate-type isozyme Homo sapiens
-
neuron pyramidal, hippocampus Homo sapiens
-
prostate especially expressed in secretory epithelial cells Homo sapiens
-
prostate prostate-type isozyme, secreted into the medium Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Arg-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens acetyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O best substrate Homo sapiens acetyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens acetyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens Arg + 7-amino-4-methylcoumarin
-
 ?
benzyl-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens benzyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens benzyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens benzyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Leu-Lys-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Pro-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
Pro-Phe-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Pyr-Gly-Arg-4-methyl-coumaryl-7-amide + H2O recombinant enzyme Homo sapiens Pyr-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Homo sapiens
? x * 44000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
kallikrein-like protease
-
 Homo sapiens
TLSP
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens