Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
benzyloxycarbonyl-Arg p-nitrobenzyl ester | - |
Homo sapiens | |
5.2 | - |
benzyloxycarbonyl-L-Lys-thiobenzyl ester | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49863 | - |
- |
Purification (Comment) | Organism |
---|---|
DE52 anion-exchange column chromatography, Q Sepharose column chromatography, hydroxylapatite column chromatography, and Sephadex G-75 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cytotoxic T-cell | - |
Homo sapiens | - |
natural killer cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0529 | - |
cell extract, using benzyloxycarbonyl-Lys-thiobenzyl ester as a substrate, at 25°C | Homo sapiens |
37.1 | - |
after 1202.9fold purification, using benzyloxycarbonyl-Arg p-nitrobenzyl ester as a substrate, at 25°C | Homo sapiens |
63.7 | - |
after 1202.9fold purification, using benzyloxycarbonyl-Lys-thiobenzyl ester as a substrate, at 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg p-nitrobenzyl ester + H2O | - |
Homo sapiens | benzyloxycarbonyl-Arg + p-nitrobenzyl alcohol | - |
? | |
benzyloxycarbonyl-L-Lys-thiobenzyl ester + H2O | - |
Homo sapiens | benzyloxycarbonyl-L-Lys + thiobenzyl alcohol | - |
? | |
Cbz-L-Arg-4-nitroanilide + H2O | little hydrolyzed by Gr3 | Homo sapiens | Cbz-L-Arg + 4-nitroaniline | - |
? | |
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O | little hydrolyzed by Gr3 | Homo sapiens | Cbz-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
Cbz-L-Lys-4-nitroanilide + H2O | little hydrolyzed by Gr3 | Homo sapiens | Cbz-L-Lys + 4-nitroaniline | - |
? | |
fluorescence resonance energy transfer substrate-25Arg peptide + H2O | most effectively hydrolyzes the substrate fluorescence resonance energy transfer substrate-25Arg peptide in the FRETS-25Xaa series tested | Homo sapiens | ? | - |
? | |
fluorescence resonance energy transfer substrate-25Lys peptide + H2O | far less effectively cleaved than fluorescence resonance energy transfer substrate-25Arg peptide | Homo sapiens | ? | - |
? | |
additional information | most efficiently hydrolyzes the carboxylic side of Phe-Tyr-Arg (P3-P2-P1) sequence | Homo sapiens | ? | - |
? | |
Z-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-Leu-Arg + Gly-Gly-7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gr3 | - |
Homo sapiens |
granzyme 3 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Homo sapiens |