Literature summary for 3.4.21.B34 extracted from

Bosch, J.; Tamura, T.; Tamura, N.; Baumeister, W.; Essen, L.O.
The beta-propeller domain of the trilobed protease from Pyrococcus furiosus reveals an open Velcro topology (2007), Acta Crystallogr. Sect. D, D63, 179-187.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)-RIL cells	Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, with 100 mM sodium acetate pH 4.4, 20 mM MgCl2, 300 mM 1,6-hexanediol and 10% (w/v) PEG 4000	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
220000	-	SDS-PAGE	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Pyrococcus furiosus

Subunits

Subunits	Comment	Organism
trimer	x-ray crystallography	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
TLP	-	Pyrococcus furiosus
trilobed protease	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)