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Literature summary for 3.4.21.B34 extracted from

  • Tamura, T.; Tamura, N.; Cejka, Z.; Hegerl, R.; Lottspeich, F.; Baumeister, W.
    Tricorn protease--the core of a modular proteolytic system (1996), Science, 274, 1385-1389.
    View publication on PubMed
Search for more literature for 3.4.21.B34

Inhibitors

Inhibitors Comment Organism Structure
tosyl-L-phenylalanine chloromethyl ketone
-
 Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 6 * 120000, the hexameric toroid can assemble further into a capsid structure. Tricorn protease appears to act as a core of a proteolytic system, when it interacts with several smaller proteins, it displays multicatalytic activities, SDS-PAGE Thermoplasma acidophilum
720000
-
 gel filtration Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum P96086
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Thermoplasma acidophilum ?
-
 ?
benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Thermoplasma acidophilum ?
-
 ?
benzyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Thermoplasma acidophilum ?
-
 ?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Thermoplasma acidophilum ?
-
 ?
additional information no cleavage of succinyl-Leu-Tyr-7-amido-4-methylcoumarin, succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin and benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin Thermoplasma acidophilum ?
-
 ?
tert-butyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Thermoplasma acidophilum ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 120000, the hexameric toroid can assemble further into a capsid structure. Tricorn protease appears to act as a core of a proteolytic system, when it interacts with several smaller proteins, it displays multicatalytic activities, SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 8.8
-
 Thermoplasma acidophilum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoplasma acidophilum calculation from nucleotide sequence
-
 5.8