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Literature summary for 3.4.21.B34 extracted from

  • Tamura, N.; Pfeifer, G.; Baumeister, W.; Tamura, T.
    Tricorn protease in bacteria: characterization of the enzyme from Streptomyces coelicolor (2001), Biol. Chem., 382, 449-458.
    View publication on PubMed
Search for more literature for 3.4.21.B34

Cloned(Commentary)

Cloned (Comment) Organism
expression of Tri-SCC77 in Escherichia coli Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
Ala-Ala-Phe-chloromethylketone 500 nM, 90% inhibition Streptomyces coelicolor
Ca2+ 100 mM, CaCl2, 57% inhibition Streptomyces coelicolor
carbobenzoxy-L-Leu-L-Leu-L-leucinal i.e. MG-132, 0.2 mM, 64% inhibition Streptomyces coelicolor
NEM 1 mM, enzyme retains 87% of its activity Streptomyces coelicolor
tosyl-L-phenylalanine-chloromethylketone 0.2 mM, 90% inhibition Streptomyces coelicolor
Zn2+ 25 mM ZnCl2, 60% inhibition Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 100 mM KCl, enhances activity to 121% of control Streptomyces coelicolor
Mg2+ 25 mM MgCl2, 7fold enhancement of peptidase activity Streptomyces coelicolor
Mn2+ 25 mM MnCl2, enhances activity to 141% of the control Streptomyces coelicolor
Na+ 100 mM NaCl, enhances activity to 132% of control Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
 6 * 117000, SDS-PAGE Streptomyces coelicolor
700000
-
 gel filtration Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9RDE2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Streptomyces coelicolor Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O 5.5% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor benzoyl-Val-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O 11% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 5.5% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O 1% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 22% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 1% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O 75% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O 2% of the activity with Ala-Ala-Phe-7-amido-4-methylcoumarin Streptomyces coelicolor tert-butyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 117000, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
Tri-ScC77
-
 Streptomyces coelicolor
Tricorn protease homolog 1
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8
-
 Streptomyces coelicolor