Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RecA | required for activity, RecA facilitates the intermolecular selfcleavage of UmuD2 homodimer | Escherichia coli | |
RecA | UmuDAb requires RecA for cleavage | Acinetobacter baylyi |
Cloned (Comment) | Organism |
---|---|
gene umuD | Escherichia coli |
gene umuDAb, the umuD homologue umuDAb is present throughout the Acinetobacter genus and encodes an extra N-terminal region, transformation of Escherichia coli wild-type strain AB1157 and mutant cells with plasmids bearing various umuDAb alleles. UmuD is not required for UmuDAb expression from its native promoter, nor its disappearance after DNA damage through intermolecular interactions with Escherichia coli UmuD | Acinetobacter baylyi |
Protein Variants | Comment | Organism |
---|---|---|
A83X | the site-directed mutation of UmuDAb at Ala83 abolishes cleavage activity | Acinetobacter baylyi |
K156X | the site-directed mutation of UmuDAb at Lys156 abolishes cleavage activity | Acinetobacter baylyi |
additional information | generation of DELTAumuD mutant cells | Escherichia coli |
additional information | generation of DELTAumuD mutant cells | Acinetobacter baylyi |
S119X | the site-directed mutation of UmuDAb at Ser119 abolishes cleavage activity | Acinetobacter baylyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
2 * 15000, recombinant enzyme UmuD, SDS-PAGE | Escherichia coli |
24000 | - |
x * 24000, recombinant enzyme variant UmuDAb, SDS-PAGE | Acinetobacter baylyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UmuDAb + H2O | Acinetobacter baylyi | slow auto-cleavage of UmuDAb to UmuDAb'. UmuDAb undergoes a post-translational, LexA-like cleavage event after DNA damage, possibly to achieve its regulatory action | UmuDAb' + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | - |
gene umuDAb, encoded in the umuDC operon | - |
Escherichia coli | - |
gene umuD, encoded in the umuDC operon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UmuD + H2O | auto-cleavage of UmuD to UmuD' | Escherichia coli | UmuD' + ? | - |
? | |
UmuDAb + H2O | slow auto-cleavage of UmuDAb to UmuDAb'. UmuDAb undergoes a post-translational, LexA-like cleavage event after DNA damage, possibly to achieve its regulatory action | Acinetobacter baylyi | UmuDAb' + ? | - |
? | |
UmuDAb + H2O | slow auto-cleavage of UmuDAb to UmuDAb', cleavage site is Ala83-Gly84 | Acinetobacter baylyi | UmuDAb' + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, recombinant enzyme variant UmuDAb, SDS-PAGE | Acinetobacter baylyi |
homodimer | 2 * 15000, recombinant enzyme UmuD, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
error-prone polymerase accessory | - |
Escherichia coli |
UmuD | - |
Escherichia coli |
UmuDAb | - |
Acinetobacter baylyi |
General Information | Comment | Organism |
---|---|---|
malfunction | effects of recA and umuD mutations on UmuDAb cleavage in DNA damage response of Escherichia coli | Escherichia coli |
malfunction | effects of recA and umuD mutations on UmuDAb cleavage in DNA damage response of Escherichia coli | Acinetobacter baylyi |
additional information | Acinetobacter UmuDAb possesses both the conserved serine-lysine catalytic dyad required by UmuD, LexA, and some bacteriophage repressors for self-cleavage as well as the (Ala/Cys)-Gly cleavage site | Acinetobacter baylyi |
additional information | intermolecular mechanism of UmuD self-cleavage of enzyme dimers, overview | Escherichia coli |
physiological function | in the DNA damage response, UmuD forms part of the error-prone (UmuD'2)C polymerase V, and is activated for this function by self-cleavage after DNA damage. The umuD homologue umuDAb regulates transcription of DNA-damage induced genes. DNA damage from mitomycin C or UV exposure causes UmuDAb cleavage in both Escherichia coli wild-type and DELTAumuD cells on a timescale resembling UmuD, but does not require UmuD. UmuD and UmuDAb require RecA for cleavage | Acinetobacter baylyi |
physiological function | when bound to UmuC, the enzyme functions as a checkpoint in delaying cell division, allowing time for error-free repair mechanisms to act, error-prone polymerase accessory UmuD. UmuD is not required for UmuDAb expression from its native promoter, nor its disappearance after DNA damage through intermolecular interactions with Escherichia coli UmuD | Escherichia coli |