Literature summary for 3.4.21.B30 extracted from

Beuning Penny , B.P.; Simon Sharotka , S.S.; Zemla Ada, Z.A.; Barsky Danie, B.D.; Walker Graham , W.G.
A non-cleavable UmuD variant that acts as a UmuD mimic (2006), J. Biol. Chem., 281, 9633-9640.

Search for more literature for 3.4.21.B30

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
F15A	slight decrease of induced mutagenesis compared to wild-type	Escherichia coli
F18A	decrease of induced mutagenesis to 20% of wild-type level, no cleavage of UmuD	Escherichia coli
T14A	slight decrease of induced mutagenesis compared to wild-type	Escherichia coli
T14A/F15A/F18A	decrease of induced mutagenesis to 20% of wild-type level, no cleavage of UmuD	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AG11	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-tag fusion protein, Ni-affinity chromatography	Escherichia coli

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UmuD + H2O	UmuD undergoes cleavage upon interaction with a RecA/ssDNA complex	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
UmuD	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)