Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.B3 extracted from

  • Sokolova, E.A.; Starkova, N.N.; Vorotyntseva, T.I.; Zamolodchikova, T.S.
    A serine protease from the bovine duodenal mucosa, chymotrypsin-like duodenase (1998), Eur. J. Biochem., 255, 501-507.
    View publication on PubMed
Search for more literature for 3.4.21.B3

General Stability

General Stability Organism
methanol 1% v/v stabilizes the purified enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
alpha-N-tosyl-L-lysine chloromethane
-
 Bos taurus
Bowman Birk soybean inhibitor
-
 Bos taurus
chymostatin
-
 Bos taurus
chymotrypsin inhibitor form I and II from potato, from kidney bean Bos taurus
diisopropylphosphofluoridate complete inhibition, irreversible inhibition Bos taurus
EDTA weak inhibition at high concentration Bos taurus
o-phenanthroline weak Bos taurus
phenylmethanesulfonyl fluoride irreversible inhibition Bos taurus
Trypsin inhibitor from tree-thorned acacia, from kidney bean, from soybean, from Lima beans Bos taurus
white ovomucoid from chicken egg Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
1.1
-
 alpha-N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide pH 8.0, 37°C Bos taurus
1.2
-
 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
1.3
-
 alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
1.5
-
 alpha-N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24970
-
 mass spectrometry Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Bos taurus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus P80219
-
-

Purification (Commentary)

Purification (Comment) Organism
at least 90% purity Bos taurus

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro active site serine Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
 Bos taurus
-
mucosa
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.162
-
 purified enzyme Bos taurus

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, in water stable for at least 1 year, lyophilized stable for several months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ala-Ala-Phe + 4-nitroaniline
-
 ?
alpha-N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
alpha-N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Gly-Gly-Phe + 4-nitroaniline
-
 ?
alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Val-Pro-Phe + 4-nitroaniline
-
 ?
polypeptide + H2O
-
 Bos taurus peptides
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23000-24000, nonreducing SDS-PAGE, 1 * 27000-28000, reducing SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
ChlD
-
 Bos taurus
chymotrypsin-like duodenase
-
 Bos taurus
duodenase I
-
 Bos taurus
duodenase serine protease
-
 Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 10 min, in solution at neutral pH, loss of 30% activity Bos taurus
70
-
 stable up to Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 alpha-N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide pH 8.0, 37°C Bos taurus
3.6
-
 alpha-N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
13.8
-
 alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
68.4
-
 alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
168
-
 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 broad optimum at pH 8.0-10.5, maximum at pH 10.0 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 stable Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus above
-
 10