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Literature summary for 3.4.21.B3 extracted from
- Duodenase, a new serine protease of unusual specificity from bovine duodenal mucosa. Purification and properties (1995), Eur. J. Biochem., 227, 866-872.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-N-tosyl-L-lysine chloromethane | inhibitory effect on chymotrypsin-like and trypsin-like activity | Bos taurus |  |
| diisopropylphosphofluoridate | irreversible inhibition | Bos taurus | |
| inhibitor-chymotrypsin complex | no inhibition of trypsin-like and chymotrypsin-like activity by EDTA, Ca2+, and benzamidine | Bos taurus | |
| Kunitz type soybean trypsin inhibitor | inhibitory effect on chymotrypsin-like and trypsin-like activity | Bos taurus | |
| phenylmethanesulfonyl fluoride | irreversible inhibition | Bos taurus | |
| Trypsin inhibitor | from bovine lungs, from Lima beans, inhibitory effect on chymotrypsin-like and trypsin-like activity | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
FMRF-NH2 | pH 8.0, 37°C <2> | Bos taurus | |
| 0.28 | - |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 0.8 | - |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 1 | - |
LEEELKPLEEVLNL | pH 8.0, 37°C | Bos taurus | |
| 1.1 | - |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 3.2 | - |
alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 10.4 | - |
alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polypeptide + H2O | Bos taurus | enzyme belongs to the serine protease subfamily | peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P80219 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro | active site serine | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| duodenum | - |
Bos taurus | - |
| mucosa | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 53 | - |
purified enzyme, substrate Ac-Leu-Lys-Arg-4-nitroanilide | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-acetyl-Leu-Leu-Arg + 4-nitroaniline | - |
? | |
| alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-acetyl-Leu-Lys + Arg + 4-nitroaniline | - |
? | |
| alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg + 4-nitroaniline | - |
? | |
| alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg + 4-nitroaniline | - |
? | |
| alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ala-Ala-Phe + 4-nitroaniline | - |
? | |
| bovine serum albumin + H2O | enzyme cleaves the bonds Phe26-Ser27, Lys40-Leu41, Lys63-Ser64, Lys209-Ala210, Lys230-Leu231, Lys237-Val238, and Tyr409-Thr410 | Bos taurus | fragments of bovine serum albumin | - |
? | |
| FMRF-NH2 + H2O | - |
Bos taurus | ? | - |
? | |
| glucagon + H2O | enzyme cleaves the bonds Lys12-Arg13 and Arg18-Ala19 | Bos taurus | fragments of glucagon | - |
? | |
| LEEELKPLEEVLNL + H2O | - |
Bos taurus | LEEELKPL + EEVLNL | - |
? | |
| polypeptide + H2O | - |
Bos taurus | peptides | - |
? | |
| polypeptide + H2O | enzyme belongs to the serine protease subfamily | Bos taurus | peptides | - |
? | |
| porcine proinsulin + H2O | enzyme cleaves the bonds Lys-29-Thr30, Arg31-Arg32, Arg32-Glu33, Leu56-Ala57, Lys64-Arg65, and Arg66-Gly67 | Bos taurus | fragments of porcine proinsulin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| duodenase I | - |
Bos taurus |
| duodenase serine protease | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 1.2 | - |
alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 3.6 | - |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 4.2 | - |
FMRF-NH2 | pH 8.0, 37°C | Bos taurus | |
| 14.4 | - |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
| 60 | - |
LEEELKPLEEVLNL | pH 8.0, 37°C | Bos taurus | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.2 | - |
Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
Kunitz soybean trypsin inhibitor | pH 8.0, 37°C | Bos taurus |
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