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Literature summary for 3.4.21.B3 extracted from

  • Sokolova, E.A.; Mirgorodskaya, O.A.; Roepstorff, P.; Savelyeva, N.V.; Zamolodchikova, T.S.
    Comparative study of the action of bovine duodenal proteinases (duodenases) on polypeptide substrates (2001), Biochemistry (Moscow), 66, 62-67.
    View publication on PubMed
Search for more literature for 3.4.21.B3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 angiotensinogen-(1-14) pH 8.9, 37°C Bos taurus
0.03
-
 angiotensinogen-(1-14) pH 8.0, 37°C Bos taurus
0.042
-
 angiotensinogen-(1-14) pH 8.9, 37°C Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Bos taurus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus P80219
-
-

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro serine protease which has a dual specificity, i.e. both trypsin-like and chymotrypsin-like activities Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Val-Pro-Phe + 4-nitroaniline
-
 ?
alpha-N-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?
angiotensinogen-(1-14) + H2O
-
 Bos taurus fragments of angiotensinogen-(1-14)
-
 ?
glucagon + H2O
-
 Bos taurus fragments of glucagon
-
 ?
human proinsulin + H2O cleaved bonds, overview Bos taurus fragments of human proinsulin
-
 ?
melittin + H2O
-
 Bos taurus fragments of melittin
-
 ?
polypeptide + H2O
-
 Bos taurus peptides
-
 ?

Synonyms

Synonyms Comment Organism
ChlD
-
 Bos taurus
DSD
-
 Bos taurus
dual-specificity duodenase
-
 Bos taurus
duodenase I
-
 Bos taurus
duodenase serine protease
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
126
-
 angiotensinogen-(1-14) pH 8.9, 37°C Bos taurus
168
-
 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
186
-
 angiotensinogen-(1-14) pH 8.0, 37°C Bos taurus