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Literature summary for 3.4.21.B28 extracted from

  • Lee, K.; Jackson, K.; Christiansen, V.; Dolence, E.; Mckee, P.
    Enhancement of fibrinolysis by inhibiting enzymatic cleavage of precursor alpha2-antiplasmin (2011), J. Thromb. Haemost., 9, 987-996.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B28

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-D-Ala-L-boroPro selectively inhibits antiplasmin-cleaving enzyme vs. plasma dipeptidyl peptidase IV Homo sapiens
acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-D-Asp-L-boroPro
-
 Homo sapiens
acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-Gly-L-boroPro
-
 Homo sapiens
acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-Ser-Gly-L-boroPro
-
 Homo sapiens
acetyl-Arg-Gly-Gly-L-boroPro
-
 Homo sapiens
acetyl-Arg-peg-D-Asp-L-boroPro
-
 Homo sapiens
acetyl-Arg-peg-Gly-L-boroPro
-
 Homo sapiens
acetyl-Arg-peg-Ser-Gly-L-boroPro
-
 Homo sapiens
acetyl-Gly-L-boroPro
-
 Homo sapiens
Val-boroPro
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 Tic-Pro-7-amido-4-trifluoromethylcoumarin in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-L-Pro + 7-amino-4-methylcoumarin
-
 ?
precursor Met-alpha2-antiplasmin + H2O the enzyme cleaves between Pro12 and Asn13 Homo sapiens L-Asn-alpha2-antiplasmin + ?
-
 ?
Tic-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
antiplasmin-cleaving enzyme
-
 Homo sapiens
APCE
-
 Homo sapiens
FAP
-
 Homo sapiens
fibroblast activation protein
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.19
-
 Tic-Pro-7-amido-4-trifluoromethylcoumarin in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000018
-
 acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-Gly-L-boroPro in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens
0.0000027
-
 acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-Ser-Gly-L-boroPro in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens
0.0000057
-
 acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-D-Ala-L-boroPro in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens
0.0000172
-
 acetyl-Arg-Gly-Gly-L-boroPro in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens
0.0000207
-
 acetyl-Gly-L-boroPro in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens
0.001377
-
 acetyl-Arg-(8-amino-3,6-dioxaoctanoic acid)-D-Asp-L-boroPro in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
 Tic-Pro-7-amido-4-trifluoromethylcoumarin in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C Homo sapiens