Cloned (Comment) | Organism |
---|---|
into a modified pFastBacHTb vector | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
at 4°C, by Syrrx´s automated Nanovolume crystallization technology, Ala657 reduces the acidity in this pocket, which may explain the lower affinity for N-terminal amines | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A657D | shows a 60fold increase in the catalytic efficiency for the cleavage of dipeptide substrates, catalytic efficiency reduced by ca. 350fold for the cleavage of benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
theoretical value of FAPalpha-His6tag | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
standard protocol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FAPalpha | - |
Homo sapiens |