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Literature summary for 3.4.21.B27 extracted from

  • Munzer, J.S.; Basak, A.; Zhong, M.; Mamarbachi, A.; Hamelin, J.; Savaria, D.; Lazure, C.; Hendy, G.N.; Benjannet, S.; Chretien, M.; Seidah, N.G.
    In vitro characterization of the novel proprotein convertase PC7 (1997), J. Biol. Chem., 272, 19672-19681.
    View publication on PubMed
Search for more literature for 3.4.21.B27

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and truncated form Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 97% inhibition at 1 mM Rattus norvegicus
EDTA 95% inhibition at 10 mM Rattus norvegicus
ZnSO4 94% inhibition at 1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.164
-
 pERTKR-4-methylcoumarin 7-amide pH 6.5 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Rattus norvegicus 5783
-
membrane mature enzyme Rattus norvegicus 16020
-
soluble truncated enzyme Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent, optimal concentration: 1 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 SDS-PAGE, truncated enzyme Rattus norvegicus
89000
-
 SDS-PAGE, mature enzyme Rattus norvegicus
101000
-
 SDS-PAGE, zymogen Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein zymogen is N-glycosylated Rattus norvegicus
proteolytic modification zymogen is processed to mature enzyme in the endoplasmic reticulum Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
KEKR-4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus KEKR + 7-amino-4-methylcoumarin
-
 ?
pERTKR-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus pERTKR + 7-amino-4-methylcoumarin
-
 ?
pro-parathyroid hormone tridecapeptide + H2O
-
 Rattus norvegicus ?
-
 ?
pro-PC4 heptadecapeptide + H2O
-
 Rattus norvegicus ?
-
 ?
RFAR-4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus RFAR + 7-amino-4-methylcoumarin
-
 ?
RKKR-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus RKKR + 7-amino-4-methylcoumarin
-
 ?
RSKR-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus RSKR + 7-amino-4-methylcoumarin
-
 ?
RSKR-4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus RSKR + 7-amino-4-methylcoumarin
-
 ?
RVRR-4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus RVRR + 7-amino-4-methylcoumarin
-
 ?
YEKERSKR-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus YEKERSKR + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
rPC7
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Rattus norvegicus