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Literature summary for 3.4.21.B21 extracted from

  • Uitto, V.
    Trepolisin (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , Eds. ) Academic Press, 2, 1813-1815.
No PubMed abstract available
Search for more literature for 3.4.21.B21

Activating Compound

Activating Compound Comment Organism Structure
cysteine increases activity Treponema denticola
DTT increases activity Treponema denticola

Application

Application Comment Organism
additional information contributes to tissue invasion and lysis of host cells, important in migration of the organism through basement membrane, cytopathic effects on epithelial cells, able to trigger release of polymorphonuclear leukocyte granules and activates latent matrix metalloproteinases Treponema denticola

Inhibitors

Inhibitors Comment Organism Structure
Bowman-Birk soybean trypsin-chymotrypsin inhibitor
-
 Treponema denticola
DFP inactivates Treponema denticola
diethyl dicarbonate inactivates Treponema denticola
additional information not inhibited by Tos-Lys-CH2Cl, alpha1-proteinase inhibitor and pepstatin Treponema denticola
N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline inactivates Treponema denticola
PMSF
-
 Treponema denticola
thiol-blocking reagent
-
 Treponema denticola
Tos-Phe-CH2Cl
-
 Treponema denticola

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane
-
 Treponema denticola 19867
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases activity somewhat or has no effect Treponema denticola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
-
 Treponema denticola

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
 ATCC strains 33520 and 35405
-

Purification (Commentary)

Purification (Comment) Organism
rapidly purified by sonication, SDS-PAGE, gel filtration, pressure filtration, 101fold purified by ion-exchange chromatography followed by gel filtration Treponema denticola

Renatured (Commentary)

Renatured (Comment) Organism
heating at 100°C results in formation of three polypeptides Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-proteinase inhibitor + H2O
-
 Treponema denticola ?
-
 ?
angiotensin + H2O
-
 Treponema denticola ?
-
 ?
casein + H2O
-
 Treponema denticola ?
-
 ?
Fibrinogen + H2O
-
 Treponema denticola ?
-
 ?
Fibronectin + H2O
-
 Treponema denticola ?
-
 ?
Gelatin + H2O
-
 Treponema denticola ?
-
 ?
IgA + H2O
-
 Treponema denticola ?
-
 ?
IgG + H2O
-
 Treponema denticola ?
-
 ?
Laminin + H2O
-
 Treponema denticola ?
-
 ?
additional information no degradation of native type I collagen Treponema denticola ?
-
 ?
serum albumin + H2O
-
 Treponema denticola ?
-
 ?
Substance P + H2O
-
 Treponema denticola ?
-
 ?
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Treponema denticola Suc-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?
transferrin + H2O
-
 Treponema denticola ?
-
 ?
Type IV collagen + H2O
-
 Treponema denticola ?
-
 ?

Synonyms

Synonyms Comment Organism
chymotrypsin-like proteinase
-
 Treponema denticola
dentilisin
-
 Treponema denticola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Treponema denticola